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  Advances in the molecular detection of tuberculosis in pre-contact Andean South America

Nelson, E. A., Buikstra, J. E., Herbig, A., Tung, T. A., & Bos, K. I. (2020). Advances in the molecular detection of tuberculosis in pre-contact Andean South America. International Journey of Paleopathology, 2019.12.006. doi:http://www.sciencedirect.com/science/article/pii/S1879981719301536.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-8B3F-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-8B40-6
Genre: Journal Article

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 Creators:
Nelson, Elizabeth A.1, Author              
Buikstra, Jane E., Author
Herbig, Alexander1, Author              
Tung, Tiffiny A., Author
Bos, Kirsten I.2, Author              
Affiliations:
1Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074310              
2CoDisEASe, Max Planck Institute for the Science of Human History, Max Planck Society, ou_3033585              

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Free keywords: Andean South America, Ancient DNA, Huari, Molecular paleopathology, Tuberculosis
 Abstract: Andean paleopathological research has significantly enhanced knowledge about the geographical distribution and evolution of tuberculosis (TB) in pre-Columbian South America. In this paper, we review the history and progress of research on ancient tuberculosis (TB) in the Andean region, focusing on the strengths and limitations of current approaches for the molecular detection of ancient pathogens, with special attention to TB. As a case study, we describe a molecular screening approach for the detection of ancient Mycobacterium tuberculosis in individuals from Late Intermediate Period (1000–1400 CE) contexts at the site of Huari, Peru. We evaluate 34 commingled human vertebrae and combine morphological assessments of pathology with high throughput sequencing and a non-selective approach to ancient pathogen DNA screening. Our method enabled the simultaneous detection of ancient M. tuberculosis DNA and an evaluation of the environmental microbial composition of each sample. Our results show that despite the dominance of environmental DNA, molecular signatures of M. tuberculosis were identified in eight vertebrae, six of which had no observable skeletal pathology classically associated tuberculosis infection. This screening approach will assist in the identification of candidate samples for downstream genomic analyses. The method permits higher resolution disease identification in cases where pathology may be absent, or where the archaeological context may necessitate a broad differential diagnosis based on morphology alone.

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Language(s): eng - English
 Dates: 2020-01-20
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: International Journey of Paleopathology
Source Genre: Journal
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Publ. Info: Elsevier
Pages: - Volume / Issue: - Sequence Number: 2019.12.006 Start / End Page: - Identifier: ISSN: 1879-9817
CoNE: https://pure.mpg.de/cone/journals/resource/1879-9817