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  Leaflet-dependent distribution of PtdIns[4,5]P2 in supported model membranes

Schäfer, J., Nehls, J., Schön, M., Mey, I. P., & Steinem, C. (2020). Leaflet-dependent distribution of PtdIns[4,5]P2 in supported model membranes. Langmuir, 36(5), 1320-1328. doi:10.1021/acs.langmuir.9b03793.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-8B5D-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-A27B-A
Genre: Journal Article

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Schäfer, J., Author
Nehls, J., Author
Schön, M., Author
Mey, I. P., Author
Steinem, C.1, Author              
Affiliations:
1Max Planck Fellow Group Membrane-based biomimetic nano- and micro-compartments, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2586691              

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 Abstract: Supported planar lipid bilayers (SLBs) prepared by spreading of unilamellar vesicles on hydrophilic substrates such as silicon dioxide are frequently used to investigate lipid-protein interactions by means of surface sensitive methods. In recent years, the receptor lipid PtdIns[4,5]P2 became particularly important as a significant number of proteins bind to this lipid at the inner leaflet of the plasma membrane. Here, we investigated how the lipid PtdIns[4,5]P2 distributes between the two leaflets of an SLB on SiO2 surfaces. We prepared SLBs on SiO2 by spreading small unilamellar vesicles and quantified the adsorption of PtdIns[4,5]P2 binding proteins providing information about the accessibility of PtdIns[4,5]P2. We compared protein binding to PtdIns[4,5]P2 in SLBs with that in lipid monolayers on a 1,1,1-trimethyl-N-(trimethylsilyl)silanamine-functionalized SiO2 surface using reflectometric interference spectroscopy and atomic force microscopy. Our results clearly demonstrate that the accessibility of PtdIns[4,5]P2 for protein binding is reduced in SLBs compared to that in supported hybrid membranes, which is discussed in terms of PtdIns[4,5]P2 distribution in the two leaflets of SLBs.

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Language(s): eng - English
 Dates: 2020-01-172020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acs.langmuir.9b03793
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Title: Langmuir
Source Genre: Journal
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Pages: - Volume / Issue: 36 (5) Sequence Number: - Start / End Page: 1320 - 1328 Identifier: -