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  Small- and large-scale high molecular weight genomic DNA extraction from planarians.

Grohme, M. A., Vila Farre, M., & Rink, J. C. (2018). Small- and large-scale high molecular weight genomic DNA extraction from planarians. In J. C. Rink (Ed.), Planarian Regeneration: Methods and Protocols. Totowa, USA: Humana Press.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0005-8BBE-9 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-0957-8
Genre: Book Chapter

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 Creators:
Grohme, M. A., Author
Vila Farre, M.1, Author           
Rink, J. C.1, Author           
Affiliations:
1Department of Tissue Dynamics and Regeneration, MPI for Biophysical Chemistry, Max Planck Society, ou_3181978              

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Free keywords: Planarian; Genomic DNA; Chaotropic salt; CTAB; Organic phase extraction; Next-generation sequencing
 Abstract: High-quality genomic DNA extraction is a starting point for many downstream applications in modern molecular biology. Here, we describe a simple method for isolating high molecular weight genomic DNA from planarians. The method is based on tissue lysis by a mixture of a chaotropic salt and detergent followed by organic extraction to remove proteins and lipids followed by a postpurification step to remove contaminating polysaccharides. The isolated DNA is of high molecular weight and compatible with polymerase chain reaction, cloning, or next-generation sequencing library preparation.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4939-7802-1_7
 Degree: -

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Title: Planarian Regeneration: Methods and Protocols
Source Genre: Book
 Creator(s):
Rink, J. C.1, Editor           
Affiliations:
1 Department of Tissue Dynamics and Regeneration, MPI for Biophysical Chemistry, Max Planck Society, ou_3181978            
Publ. Info: Totowa, USA : Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: -

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Title: Methods in Molecular Biology
Source Genre: Series
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Publ. Info: -
Pages: - Volume / Issue: 1774 Sequence Number: - Start / End Page: 267 - 275 Identifier: -