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  Controlled division of cell-sized vesicles by low densities of membrane-bound proteins

Steinkühler, J., Knorr, R. L., Zhao, Z., Bhatia, T., Bartelt, S. M., Wegner, S., et al. (2020). Controlled division of cell-sized vesicles by low densities of membrane-bound proteins. Nature Communications, 11: 905. doi:10.1038/s41467-020-14696-0.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-8D59-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-AE9C-8
Genre: Journal Article

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 Creators:
Steinkühler, Jan1, Author              
Knorr, Roland L.2, Author              
Zhao, Ziliang1, Author              
Bhatia, Tripta1, Author              
Bartelt, Solveig M., Author
Wegner, Seraphine, Author
Dimova, Rumiana1, Author              
Lipowsky, Reinhard3, Author              
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              
2Roland Knorr, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2288692              
3Reinhard Lipowsky, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863327              

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Free keywords: Biological physics; biophysical chemistry; membrane biophysics; synthetic biology
 Abstract: The proliferation of life on earth is based on the ability of single cells to divide into two daughter cells. During cell division, the plasma membrane undergoes a series of morphological transformations which ultimately lead to membrane fission. Here, we show that analogous remodeling processes can be induced by low densities of proteins bound to the membranes of cell-sized lipid vesicles. Using His-tagged fluorescent proteins, we are able to precisely control the spontaneous curvature of the vesicle membranes. By fine-tuning this curvature, we obtain dumbbell-shaped vesicles with closed membrane necks as well as neck fission and complete vesicle division. Our results demonstrate that the spontaneous curvature generates constriction forces around the membrane necks and that these forces can easily cover the force range found in vivo. Our approach involves only one species of membrane-bound proteins at low densities, thereby providing a simple and extendible module for bottom-up synthetic biology.

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Language(s): eng - English
 Dates: 2020-01-272020-02-142020
 Publication Status: Published in print
 Pages: -
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 Rev. Method: -
 Identifiers: DOI: 10.1038/s41467-020-14696-0
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 11 Sequence Number: 905 Start / End Page: - Identifier: ISSN: 2041-1723