English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Systems-Wide Analysis of Acclimation Responses to Long-Term Heat Stress and Recovery in the Photosynthetic Model Organism Chlamydomonas reinhardtii

Hemme, D., Veyel, D., Mühlhaus, T., Sommer, F., Jueppner, J., Unger, A.-K., et al. (2014). Systems-Wide Analysis of Acclimation Responses to Long-Term Heat Stress and Recovery in the Photosynthetic Model Organism Chlamydomonas reinhardtii. The Plant Cell, 26(11), 4270-4297. doi:10.1105/tpc.114.130997.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-8EAF-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-8EB0-4
Genre: Journal Article

Files

show Files

Locators

show
hide
Locator:
Link (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Hemme, D.1, Author              
Veyel, D.1, Author              
Mühlhaus, T.1, Author              
Sommer, F.1, Author              
Jueppner, J.2, Author              
Unger, Ann-Katrin3, Author
Sandmann, Michael3, Author
Fehrle, I.4, Author              
Schönfelder, S.1, Author              
Steup, Martin3, Author
Geimer, Stefan3, Author
Kopka, J.4, Author              
Giavalisco, P.2, Author              
Schroda, M.1, Author              
Affiliations:
1Plant Molecular Chaperone Networks and Stress, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753312              
2Experimental Systems Biology, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753342              
3External Organizations, ou_persistent22              
4Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              

Content

show
hide
Free keywords: -
 Abstract: We applied a top-down systems biology approach to understand how Chlamydomonas reinhardtii acclimates to long-term heat stress (HS) and recovers from it. For this, we shifted cells from 25 to 42°}C for 24 h and back to 25{°C for >=8 h and monitored abundances of 1856 proteins/protein groups, 99 polar and 185 lipophilic metabolites, and cytological and photosynthesis parameters. Our data indicate that acclimation of Chlamydomonas to long-term HS consists of a temporally ordered, orchestrated implementation of response elements at various system levels. These comprise (1) cell cycle arrest; (2) catabolism of larger molecules to generate compounds with roles in stress protection; (3) accumulation of molecular chaperones to restore protein homeostasis together with compatible solutes; (4) redirection of photosynthetic energy and reducing power from the Calvin cycle to the de novo synthesis of saturated fatty acids to replace polyunsaturated ones in membrane lipids, which are deposited in lipid bodies; and (5) when sinks for photosynthetic energy and reducing power are depleted, resumption of Calvin cycle activity associated with increased photorespiration, accumulation of reactive oxygen species scavengers, and throttling of linear electron flow by antenna uncoupling. During recovery from HS, cells appear to focus on processes allowing rapid resumption of growth rather than restoring pre-HS conditions.GlossaryHSheat stressERendoplasmic reticulumPSIIphotosystem IITAGtriacylglycerolPLBprolamellar bodyLC-MSliquid chromatography-mass spectrometryLHClight-harvesting complexPSIphotosystem IGC-MSgas chromatography-mass spectrometryGPGglycerophosphoglycerolDGTSdiacylglycerol trimethyl homoserinePEphosphatidyl ethanolamineMGDGmonogalactosyl diacylglycerolDGDGdigalactosyl diacylglycerolSQDGsulfoquinovosyl diacylglycerolPGphosphatidyl glycerolDAGdiacylglycerolFAfatty acidTCAtricarboxylic acidROSreactive oxygen speciesBCAAbranched-chain amino acidLEFlinear electron flowTAPTris-acetate-phosphatePIpropidium diiodideIOMIQSintegration of mass spectrometry identification and quantification software

Details

show
hide
Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1105/tpc.114.130997
BibTex Citekey: Hemme4270
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 26 (11) Sequence Number: - Start / End Page: 4270 - 4297 Identifier: ISSN: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X