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  Allosteric regulation through a switch element in the autophagy E2, Atg3

Qiu, Y., Zheng, Y., Grace, C. R. R., Liu, X., Klionsky, D. J., & Schulman, B. A. (2020). Allosteric regulation through a switch element in the autophagy E2, Atg3. AUTOPHAGY, 16(1), 183-184. doi:10.1080/15548627.2019.1688550.

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 Urheber:
Qiu, Yu1, Autor
Zheng, Yumei1, Autor
Grace, Christy R. R.1, Autor
Liu, Xu1, Autor
Klionsky, Daniel J.1, Autor
Schulman, Brenda A.2, Autor           
Affiliations:
1external, ou_persistent22              
2Schulman, Brenda / Molecular Machines and Signaling, Max Planck Institute of Biochemistry, Max Planck Society, ou_2466699              

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Schlagwörter: Cell Biology; Allosteric; Atg3; Atg8; autophagy; lipidation;
 Zusammenfassung: Lipidation of Atg8-family ubiquitin-like proteins (UBLs) plays important roles in macroautophagy/autophagy. This process is catalyzed by an E1-E2-E3 trienzyme cascade, in which an E1 enzyme, Atg7, directs Atg8 to its E2 enzyme, Atg3, forming a thioester bond-linked Atg3 similar to Atg8 intermediate; then the composite E3, Atg12-Atg5-Atg16, interacts with the Atg3 similar to Atg8 intermediate and promotes Atg8 transfer from the catalytic cysteine of Atg3 to the head group of phosphatidylethanolamine (PE) lipids. Despite progress that has been made toward understanding the Atg8 lipidation pathway, the molecular mechanism of Atg3 as it orchestrates between the E1 and E3 remains unclear. Here we summarize our recent work reporting an element in Atg3, termed the E1, E2, and E3-interacting region (E123IR), is an allosteric switch: in the absence of other binding partners, the E123IR restrains Atg3 ' s catalytic loop, while the E1 or E3 enzyme directly binds this region to remove this brace and thereby conformationally activate Atg3 to elicit Atg8 lipidation in vitro and in vivo.

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Sprache(n): eng - English
 Datum: 20192020
 Publikationsstatus: Erschienen
 Seiten: 2
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000495310900001
DOI: 10.1080/15548627.2019.1688550
 Art des Abschluß: -

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Titel: AUTOPHAGY
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA : TAYLOR & FRANCIS INC
Seiten: - Band / Heft: 16 (1) Artikelnummer: - Start- / Endseite: 183 - 184 Identifikator: ISSN: 1554-8627