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  Proteomics-Based Comparative Mapping of the Secretomes of Human Brown and White Adipocytes Reveals EPDR1 as a Novel Batokine

Deshmukh, A. S., Peijs, L., Beaudry, J. L., Jespersen, N. Z., Nielsen, C. H., Ma, T., et al. (2019). Proteomics-Based Comparative Mapping of the Secretomes of Human Brown and White Adipocytes Reveals EPDR1 as a Novel Batokine. CELL METABOLISM, 30(5), 963-975.e7. doi:10.1016/j.cmet.2019.10.001.

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Genre: Journal Article

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 Creators:
Deshmukh, Atul S.1, Author              
Peijs, Lone2, Author
Beaudry, Jacqueline L.2, Author
Jespersen, Naja Z.2, Author
Nielsen, Carsten H.2, Author
Ma, Tao2, Author
Brunner, Andreas D.1, Author              
Larsen, Therese J.2, Author
Bayarri-Olmos, Rafael2, Author
Prabhakar, Bhargav S.2, Author
Helgstrand, Charlotte2, Author
Severinsen, Mai C. K.2, Author
Holst, Birgitte2, Author
Kjaer, Andreas2, Author
Tang-Christensen, Mads2, Author
Sanfridson, Annika2, Author
Garred, Peter2, Author
Prive, Gilbert G.2, Author
Pedersen, Bente K.2, Author
Gerhart-Hines, Zachary2, Author
Nielsen, Soren2, AuthorDrucker, Daniel J.2, AuthorMann, Matthias1, Author              Scheele, Camilla2, Author more..
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

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Free keywords: INDUCED INSULIN-RESISTANCE; ADIPOSE-TISSUE; ENERGY-EXPENDITURE; ALPHA CONTROLS; FAT; QUANTIFICATION; THERMOGENESIS; HOMEOSTASIS; ASPROSIN; DISTINCTCell Biology; Endocrinology & Metabolism;
 Abstract: Adipokines secreted from white adipose tissue play a role in metabolic crosstalk and homeostasis, whereas the brown adipose secretome is less explored. We performed high-sensitivity mass-spectrometry-based proteomics on the cell media of human adipocytes derived from the supraclavicular brown adipose and from the subcutaneous white adipose depots of adult humans. We identified 471 potentially secreted proteins covering interesting categories such as hormones, growth factors, extracellular matrix proteins, and proteins of the complement system, which were differentially regulated between brown and white adipocytes. A total of 101 proteins were exclusively quantified in brown adipocytes, and among these was ependymin-related protein 1 (EPDR1). EPDR1 was detected in human plasma, and functional studies suggested a role for EPDR1 in thermogenic determination during adipogenesis. In conclusion, we report substantial differences between the secretomes of brown and white human adipocytes and identify novel candidate batokines that can be important regulators of human metabolism.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: CELL METABOLISM
Source Genre: Journal
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Publ. Info: 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 30 (5) Sequence Number: - Start / End Page: 963 - 975.e7 Identifier: ISSN: 1550-4131