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  An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions

Schueder, F., Stein, J., Stehr, F., Auer, A., Sperl, B., Strauss, M. T., et al. (2019). An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions. Nature Methods, 16(11), 1101-1104. doi:10.1038/s41592-019-0584-7.

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 Creators:
Schueder, Florian1, Author              
Stein, Johannes2, Author              
Stehr, Florian2, Author              
Auer, Alexander1, Author              
Sperl, Bianca1, Author              
Strauss, Maximilian T.1, Author              
Schwille, Petra2, Author              
Jungmann, Ralf1, Author              
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: LOCALIZATION MICROSCOPY; HYBRIDIZATION KINETICSBiochemistry & Molecular Biology;
 Abstract: DNA points accumulation in nanoscale topography (DNA-PAINT) is a relatively easy-to-implement super-resolution technique. However, image acquisition is slow compared to most other approaches. Here, we overcome this limitation by designing optimized DNA sequences and buffer conditions. We demonstrate our approach in vitro with DNA origami and in situ using cell samples, and achieve an order of magnitude faster imaging speeds without compromising image quality or spatial resolution. This improvement now makes DNA-PAINT applicable to high-throughput studies.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000493902600020
DOI: 10.1038/s41592-019-0584-7
 Degree: -

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Project name : Emmy Noether Program (no. DFG JU 2957/1-1), the SFB1032 (project no. A11)
Grant ID : -
Funding program : -
Funding organization : German Research Foundation
Project name : MolMap
Grant ID : 680241
Funding program : ERC Starting Grant
Funding organization : European Research Council

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Title: Nature Methods
  Other : Nature Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 16 (11) Sequence Number: - Start / End Page: 1101 - 1104 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556