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  MamY is a membrane-bound protein that aligns magnetosomes and the motility axis of helical magnetotactic bacteria

Toro-Nahuelpan, M., Giacomelli, G., Raschdorf, O., Borg, S., Plitzko, J. M., Bramkamp, M., et al. (2019). MamY is a membrane-bound protein that aligns magnetosomes and the motility axis of helical magnetotactic bacteria. NATURE MICROBIOLOGY, 4(11), 1978-1989. doi:10.1038/s41564-019-0512-8.

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Toro-Nahuelpan, Mauricio1, Author              
Giacomelli, Giacomo2, Author
Raschdorf, Oliver1, Author              
Borg, Sarah2, Author
Plitzko, Jürgen M.1, Author              
Bramkamp, Marc2, Author
Schueler, Dirk2, Author
Mueller, Frank-Dietrich2, Author
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2external, ou_persistent22              

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Free keywords: DOMAIN; EXPRESSION; BIOMINERALIZATION; LOCALIZATION; MICROSCOPY; DIVISION; DELETION; BINDING; SYSTEM; SINGLEMicrobiology;
 Abstract: To navigate within the geomagnetic field, magnetotactic bacteria synthesize magnetosomes, which are unique organelles consisting of membrane-enveloped magnetite nanocrystals. In magnetotactic spirilla, magnetosomes become actively organized into chains by the filament-forming actin-like MamK and the adaptor protein MamJ, thereby assembling a magnetic dipole much like a compass needle. However, in Magnetospirillum gryphiswaldense, discontinuous chains are still formed in the absence of MamK. Moreover, these fragmented chains persist in a straight conformation indicating undiscovered structural determinants able to accommodate a bar magnet-like magnetoreceptor in a helical bacterium. Here, we identify MamY, a membrane-bound protein that generates a sophisticated mechanical scaffold for magnetosomes. MamY localizes linearly along the positive inner cell curvature (the geodetic cell axis), probably by self-interaction and curvature sensing. In a mamY deletion mutant, magnetosome chains detach from the geodetic axis and fail to accommodate a straight conformation coinciding with reduced cellular magnetic orientation. Codeletion of mamKY completely abolishes chain formation, whereas on synthetic tethering of magnetosomes to MamY, the chain configuration is regained, emphasizing the structural properties of the protein. Our results suggest MamY is membrane-anchored mechanical scaffold that is essential to align the motility axis of magnetotactic spirilla with their magnetic moment vector and to perfectly reconcile magnetoreception with swimming direction.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000493113000024
DOI: 10.1038/s41564-019-0512-8
 Degree: -

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Title: NATURE MICROBIOLOGY
Source Genre: Journal
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Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 4 (11) Sequence Number: - Start / End Page: 1978 - 1989 Identifier: ISSN: 2058-5276