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  Sleep-wake cycles drive daily dynamics of synaptic phosphorylation

Brüning, F., Noya, S. B., Bange, T., Koutsouli, S., Rudolph, J. D., Tyagarajan, S., et al. (2019). Sleep-wake cycles drive daily dynamics of synaptic phosphorylation. SCIENCE, 366(6462): eaav3617, pp. 201. doi:10.1126/science.aav3617.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-9911-B Version Permalink: http://hdl.handle.net/21.11116/0000-0005-9912-A
Genre: Journal Article

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 Creators:
Brüning, Franziska1, Author              
Noya, Sara B.2, Author
Bange, Tanja2, Author
Koutsouli, Stella2, Author
Rudolph, Jan D.3, Author              
Tyagarajan, Shiva2, Author
Cox, Jürgen3, Author              
Mann, Matthias1, Author              
Brown, Steven A.2, Author
Robles, Maria S.2, Author
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              
3Cox, Jürgen / Computational Systems Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_2063284              

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Free keywords: GLYCOGEN-SYNTHASE KINASE-3; COMPUTATIONAL PLATFORM; IN-VIVO; WAKEFULNESS; INHIBITION; PLASTICITY; PROTEOMICS; HOMER1AScience & Technology - Other Topics;
 Abstract: The circadian clock drives daily changes of physiology, including sleep-wake cycles, through regulation of transcription, protein abundance, and function. Circadian phosphorylation controls cellular processes in peripheral organs, but little is known about its role in brain function and synaptic activity. We applied advanced quantitative phosphoproteomics to mouse forebrain synaptoneurosomes isolated across 24 hours, accurately quantifying almost 8000 phosphopeptides. Half of the synaptic phosphoproteins, including numerous kinases, had large-amplitude rhythms peaking at rest-activity and activity-rest transitions. Bioinformatic analyses revealed global temporal control of synaptic function through phosphorylation, including synaptic transmission, cytoskeleton reorganization, and excitatory/inhibitory balance. Sleep deprivation abolished 98% of all phosphorylation cycles in synaptoneurosomes, indicating that sleep-wake cycles rather than circadian signals are main drivers of synaptic phosphorylation, responding to both sleep and wake pressures.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 25
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000490014700034
DOI: 10.1126/science.aav3617
 Degree: -

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Title: SCIENCE
Source Genre: Journal
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Publ. Info: 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA : AMER ASSOC ADVANCEMENT SCIENCE
Pages: - Volume / Issue: 366 (6462) Sequence Number: eaav3617 Start / End Page: 201 Identifier: ISSN: 0036-8075