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  Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation

Wisniewski, J. R., & Zettl, K. (2019). Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation. DATA IN BRIEF, 26: 104530. doi:10.1016/j.dib.2019.104530.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-9213-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-9214-F
Genre: Journal Article

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 Creators:
Wisniewski, Jacek R.1, Author              
Zettl, Katharina1, Author              
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: Science & Technology - Other Topics; Proteomic sample preparation; Filter Aided Sample Preparation; Shotgun proteomics; Protein cleavage;
 Abstract: Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. These datasets accompany "Filter Aided Sample Preparation - A Tutorial" (Wisniewski, 2019). (C) 2019 The Authors. Published by Elsevier Inc.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000495079400107
DOI: 10.1016/j.dib.2019.104530
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Title: DATA IN BRIEF
Source Genre: Journal
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Publ. Info: RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS : ELSEVIER
Pages: - Volume / Issue: 26 Sequence Number: 104530 Start / End Page: - Identifier: ISSN: 2352-3409