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  A tunable refractive index matching medium for live imaging cells, tissues and model organisms.

Boothe, T., Hilbert, L., Heide, M., Berninger, L., Huttner, W. B., Zaburdaev, V., et al. (2017). A tunable refractive index matching medium for live imaging cells, tissues and model organisms. eLife, 6: e27240. doi:10.7554/eLife.27240.

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 Creators:
Boothe, T.1, Author              
Hilbert, L., Author
Heide, M., Author
Berninger, L., Author
Huttner, W. B., Author
Zaburdaev, V., Author
Vastenhouw, N. L., Author
Myers, E. W., Author
Drechsel, D. N., Author
Rink, J. C.1, Author              
Affiliations:
1Department of Tissue Dynamics and Regeneration, MPI for Biophysical Chemistry, Max Planck Society, ou_3181978              

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 Abstract: In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.

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Language(s): eng - English
 Dates: 2017-07-14
 Publication Status: Published online
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.7554/eLife.27240
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Title: eLife
Source Genre: Journal
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Pages: 15 Volume / Issue: 6 Sequence Number: e27240 Start / End Page: - Identifier: -