English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  An ER assembly line of AMPA-receptors controls excitatory neurotransmission and its plasticity

Schwenk, J., Boudkkazi, S., Kocylowski, M. K., Brechet, A., Zolles, G., Bus, T., et al. (2019). An ER assembly line of AMPA-receptors controls excitatory neurotransmission and its plasticity. Neuron, 104(4): e9, pp. 680-692. doi:10.1016/j.neuron.2019.08.033.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-9487-B Version Permalink: http://hdl.handle.net/21.11116/0000-0005-951A-6
Genre: Journal Article

Files

show Files
hide Files
:
Neuron_104_2020_680.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
Neuron_104_2020_680.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
Neuron_104_2020_680_Suppl.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
Neuron_104_2020_680_Suppl.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Schwenk, Jochen, Author
Boudkkazi, Sami, Author
Kocylowski, Maciej K., Author
Brechet, Aline, Author
Zolles, Gerd, Author
Bus, Thorsten1, Author              
Costa, Kaue, Author
Kollewe, Astrid, Author
Jordan, Johannes, Author
Bank, Julia, Author
Bildl, Wolfgang, Author
Sprengel, Rolf2, 3, 4, Author              
Kulik, Akos, Author
Roeper, Jochen, Author
Schulte, Uwe, Author
Fakler, Bernd, Author
Affiliations:
1Max Planck Research Group Behavioural Neurophysiology (Andreas T. Schaefer), Max Planck Institute for Medical Research, Max Planck Society, ou_1497722              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
3Olfaction Web, Max Planck Institute for Medical Research, Max Planck Society, ou_1497733              
4Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497741              

Content

show
hide
Free keywords: AMPA-type glutamate receptors, AMPA receptor, GluA proteins, biogenesis of ion channels, complex assembly, ER, mass spectrometry, assembly of protein complexes, learning and memory, synaptic plasticity, LTP, intellectual disability, proteomics
 Abstract: Excitatory neurotransmission and its activity-dependent plasticity are largely determined by AMPA-receptors (AMPARs), ion channel complexes whose cell physiology is encoded by their interactome. Here, we delineate the assembly of AMPARs in the endoplasmic reticulum (ER) of native neurons as multi-state production line controlled by distinct interactome constituents: ABHD6 together with porcupine stabilizes pore-forming GluA monomers, and the intellectual-disability-related FRRS1l-CPT1c complexes promote GluA oligomerization and co-assembly of GluA tetramers with cornichon and transmembrane AMPA-regulatory proteins (TARP) to render receptor channels ready for ER exit. Disruption of the assembly line by FRRS1l deletion largely reduces AMPARs in the plasma membrane, impairs synapse formation, and abolishes activity-dependent synaptic plasticity, while FRRS1l overexpression has the opposite effect. As a consequence, FRSS1l knockout mice display severe deficits in learning tasks and behavior. Our results provide mechanistic insight into the stepwise biogenesis of AMPARs in native ER membranes and establish FRRS1l as a powerful regulator of synaptic signaling and plasticity.

Details

show
hide
Language(s): eng - English
 Dates: 2019-06-282019-03-192019-08-202019-10-082019-11-20
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.neuron.2019.08.033
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Neuron
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 104 (4) Sequence Number: e9 Start / End Page: 680 - 692 Identifier: ISSN: 0896-6273
CoNE: https://pure.mpg.de/cone/journals/resource/954925560565