English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Mu opioid receptor mRNA expression in SHSY-5Y human neuroblastoma cells

Yu, X., Blake, A., Mao, X., & Chang, S. (2002). Mu opioid receptor mRNA expression in SHSY-5Y human neuroblastoma cells. Poster presented at 32nd Annual Meeting of the Society for Neuroscience (Neuroscience 2002), Orlando, FL, USA.

Item is

Files

show Files

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Yu, X1, Author              
Blake, AD, Author
Mao, X, Author
Chang, SL, Author
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Prolonged exposure to morphine down-regulates mu opioid receptors (MOR) on both undifferentiated and differentiated (retinoic acid or phorbol ester treated) SHSY-5Y cells. However, morphine pretreatment does not alter MOR receptor affinity for morphine. To investigate the molecular basis for morphine’s regulatory effects, we have developed a quantitative competitive reverse transcriptase-polymerase chain reaction (QC-RT-PCR) to quantify the amount of MOR present in SHSY-5Y cells. SHSY-5Y cells were differentiated and then incubated for 24 h with vehicle, morphine, naloxone, or both morphine and naloxone. Expression of MOR mRNA in undifferentiated SHSY-5Y cells was determined to be 369 fmol MOR mRNA/mg total RNA. Differentiation of SHSY-5Y cells with retinoic acid or phorbol ester up-regulated MOR mRNA expression by 40 \% and 78\%, respectively. A 24 hour treatment with morphine (10 mM) down-regulated MOR mRNA, an effect that was partially reversed by naloxone. Naloxone treatment alone upregulated MOR mRNA in undifferentiated, but not in RA-differentiated SHSY-5Y cells. Taken together, a significant component of SHSY-5Y cellular adaptation during chronic morphine treatment may occur at the mRNA level.

Details

show
hide
Language(s):
 Dates: 2002-11
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: -
 Degree: -

Event

show
hide
Title: 32nd Annual Meeting of the Society for Neuroscience (Neuroscience 2002)
Place of Event: Orlando, FL, USA
Start-/End Date: 2002-11-02 - 2002-11-07

Legal Case

show

Project information

show

Source 1

show
hide
Title: 32nd Annual Meeting of the Society for Neuroscience (Neuroscience 2002)
Source Genre: Proceedings
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: 248.18 Start / End Page: - Identifier: -