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  The phalloidin binding site of F-actin

Vandekerckhove, J., Deboben, A., Nassal, M., & Wieland, T. (1985). The phalloidin binding site of F-actin. EMBO Journal, 4(11), 2815-2818. doi:10.1002/j.1460-2075.1985.tb04008.x.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-99BB-C Version Permalink: http://hdl.handle.net/21.11116/0000-0005-99BC-B
Genre: Journal Article

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EMBOJ_4_1985_2815.pdf (Any fulltext), 831KB
 
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 Creators:
Vandekerckhove, Joel, Author
Deboben, Axel1, Author              
Nassal, Michael1, Author              
Wieland, Theodor1, Author              
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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Free keywords: phalloidin, F-actin, binding site
 Abstract: Tritium-containing affinity-labelling derivatives of phalloidin, an alkylating iodoacetyl compound (EAL) and a photolabile, carbene generating diazirine (PAL), have been reacted with rabbit muscle actin, the former after protection of thiol groups with N-ethylmaleimide. Labelled peptides generated by tryptic and/or thermolysin digestion were isolated by paper peptide mapping and characterized by determination of their amino acid sequences. EAL binds to methionine-119 and methionine-355; PAL binds to glutamic acid-117. These residues are located in regions with extremely conserved amino acid sequences. The cleft between the two domains of the actin monomer is suggested as the possible binding site for phalloidin.

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Language(s): eng - English
 Dates: 1985-07-261985-11-01
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 4 (11) Sequence Number: - Start / End Page: 2815 - 2818 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061