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  Selective autophagy degrades nuclear pore complexes

Lee, C.-W., Wilfling, F., Ronchi, P., Allegretti, M., Mosalaganti, S., Jentsch, S., et al. (2020). Selective autophagy degrades nuclear pore complexes. Nature Cell Biology, 22(2), 159-166. doi:10.1038/s41556-019-0459-2.

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 Creators:
Lee, Chia-Wei1, Author
Wilfling, Florian2, Author           
Ronchi, Paolo3, Author
Allegretti, Matteo4, Author
Mosalaganti, Shyamal4, Author
Jentsch, Stefan1, Author
Beck, Martin4, 5, Author           
Pfander, Boris6, Author
Affiliations:
1Molecular Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany, ou_persistent22              
2Jentsch, Stefan / Molecular Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565156              
3Electron Microscopy Core Facility (EMCF), European Molecular Biology Laboratory, Heidelberg, Germany, ou_persistent22              
4Structural and Computational Biology Unit, Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany, ou_persistent22              
5Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society, ou_3040395              
6DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Martinsried, Germany, ou_persistent22              

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 Abstract: Nuclear pore complexes (NPCs) are very large proteinaceous assemblies that consist of more than 500 individual proteins1,2. NPCs are essential for nucleocytoplasmic transport of different cellular components, and disruption of the integrity of NPCs has been linked to aging, cancer and neurodegenerative diseases3-7. However, the mechanism by which membrane-embedded NPCs are turned over is currently unknown. Here we show that, after nitrogen starvation or genetic interference with the architecture of NPCs, nucleoporins are rapidly degraded in the budding yeast Saccharomyces cerevisiae. We demonstrate that NPC turnover involves vacuolar proteases and the core autophagy machinery. Autophagic degradation is mediated by the cytoplasmically exposed Nup159, which serves as intrinsic cargo receptor and directly binds to the autophagy marker protein Atg8. Autophagic degradation of NPCs is therefore inducible, enabling the removal of individual NPCs from the nuclear envelope.

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Language(s): eng - English
 Dates: 2019-01-112019-12-172020-02-062020-02
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41556-019-0459-2
BibTex Citekey: lee_selective_2020
 Degree: -

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Title: Nature Cell Biology
  Other : 'Nat. Cell Biol.'
Source Genre: Journal
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Publ. Info: London : Springer Nature
Pages: - Volume / Issue: 22 (2) Sequence Number: - Start / End Page: 159 - 166 Identifier: ISSN: 1465-7392
CoNE: https://pure.mpg.de/cone/journals/resource/954925625310