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  Phase separation organizes the site of autophagosome formation

Fujioka, Y., Alam, J. M., Noshiro, D., Mouri, K., Ando, T., Okada, Y., et al. (2020). Phase separation organizes the site of autophagosome formation. Nature, 578(7794), 301-305. doi:10.1038/s41586-020-1977-6.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-A651-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-BFF6-F
Genre: Journal Article

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 Creators:
Fujioka, Yuko, Author
Alam, Jahangir Md., Author
Noshiro, Daisuke, Author
Mouri, Kazunari, Author
Ando, Toshio, Author
Okada, Yasushi, Author
May, Alexander I., Author
Knorr, Roland L.1, Author              
Suzuki, Kuninori, Author
Ohsumi, Yoshinori, Author
Noda, Nobuo N., Author
Affiliations:
1Roland Knorr, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2288692              

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Free keywords: Autophagy, Biological fluorescence, Post-translational modifications
 Abstract: Many biomolecules undergo liquid–liquid phase separation to form liquid-like condensates that mediate diverse cellular functions. Autophagy is able to degrade such condensates using autophagosomes—double-membrane structures that are synthesized de novo at the pre-autophagosomal structure (PAS) in yeast. Whereas Atg proteins that associate with the PAS have been characterized, the physicochemical and functional properties of the PAS remain unclear owing to its small size and fragility. Here we show that the PAS is in fact a liquid-like condensate of Atg proteins. The autophagy-initiating Atg1 complex undergoes phase separation to form liquid droplets in vitro, and point mutations or phosphorylation that inhibit phase separation impair PAS formation in vivo. In vitro experiments show that Atg1-complex droplets can be tethered to membranes via specific protein–protein interactions, explaining the vacuolar membrane localization of the PAS in vivo. We propose that phase separation has a critical, active role in autophagy, whereby it organizes the autophagy machinery at the PAS.

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Language(s): eng - English
 Dates: 2020-02-052020
 Publication Status: Published in print
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Title: Nature
  Abbreviation : Nature
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 578 (7794) Sequence Number: - Start / End Page: 301 - 305 Identifier: ISSN: 0028-0836
ISSN: 1476-4687 (online)