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  Protease-activated receptor signalling initiates alpha(5)beta(1)-integrin-mediated adhesion in non-haematopoietic cells

Spoerri, P. M., Strohmeyer, N., Sun, Z., Fässler, R., & Müller, D. J. (2020). Protease-activated receptor signalling initiates alpha(5)beta(1)-integrin-mediated adhesion in non-haematopoietic cells. NATURE MATERIALS, 19, 218-226. doi:10.1038/s41563-019-0580-4.

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 Creators:
Spoerri, Patrizia M.1, Author
Strohmeyer, Nico1, Author
Sun, Zhiqi2, Author              
Fässler, Reinhard2, Author              
Müller, Daniel J.1, Author
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1external, ou_persistent22              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

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Free keywords: INTEGRIN ACTIVATION; MYOSIN-II; FIBRONECTIN; TALIN; EXPRESSION; ADHESOME; BETA(1); IDENTIFICATION; CRISPR-CAS9; DYNAMICS
 Abstract: Haematopoietic cells and platelets employ G-protein-coupled receptors (GPCRs) to sense extracellular information and respond by initiating integrin-mediated adhesion. So far, such processes have not been demonstrated in non-haematopoietic cells. Here, we report that the activation of protease-activated receptors PAR1 and PAR2 induce multiple signalling pathways to establish alpha(5)beta(1)-integrin-mediated adhesion. First, PARs signal via G beta gamma and PI3K to alpha(5)beta(1)-integrins to adopt a talin- and kindlin-dependent high-affinity conformation, which triggers fibronectin binding and initiates cell adhesion. Then, within 60 s, PARs signal via G alpha(13), G alpha(i), ROCK and Src to strengthen the alpha(5)beta(1)-integrin-mediated adhesion. Furthermore, PAR signalling changes the abundance of numerous proteins in the adhesome assembled by alpha(5)beta(1)-integrins, including G alpha(13), vacuolar protein-sorting-associated protein 36, and band 4.1-like protein 4B or 5, and accelerates cell adhesion maturation, spreading and migration. The mechanistic insights describe how agonist binding to PAR employs GPCR and integrin-signalling pathways to initiate and regulate adhesion and to guide physiological responses of non-haematopoietic cells. As in haematopoietic cells and platelets, agonist binding to protease-activated receptors PAR1 and PAR2 in non-haematopoietic cells also triggers signalling pathways that lead to alpha(5)beta(1)-integrin-mediated cell adhesion.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000508325000006
DOI: 10.1038/s41563-019-0580-4
 Degree: -

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Title: NATURE MATERIALS
Source Genre: Journal
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Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 19 Sequence Number: - Start / End Page: 218 - 226 Identifier: ISSN: 1476-1122