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  How to cope with heavy metal ions: Cellular and proteome-level stress response to divalent copper and nickel in Halobacterium salinarum R1 planktonic and biofilm cells.

Völkel, S., Hein, S., Benker, N., Pfeifer, F., Lenz, C., & Losensky, G. (2020). How to cope with heavy metal ions: Cellular and proteome-level stress response to divalent copper and nickel in Halobacterium salinarum R1 planktonic and biofilm cells. Frontiers in Microbiology, 10: 3056. doi:10.3389/fmicb.2019.03056.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-A67B-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-A684-A
Genre: Journal Article

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 Creators:
Völkel, S., Author
Hein, S., Author
Benker, N., Author
Pfeifer, F., Author
Lenz, C.1, Author              
Losensky, G., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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Free keywords: microbial communities; metal stress; extracellular polymeric substances; adhesion; scanning electron microscopy; proteome; SWATH-MS; label-free quantification
 Abstract: Halobacterium salinarum R1 is an extremely halophilic archaeon capable of adhesion and forming biofilms, allowing it to adjust to a range of growth conditions. We have recently shown that living in biofilms facilitates its survival under Cu2+ and Ni2+ stress, with specific rearrangements of the biofilm architecture observed following exposition. In this study, quantitative analyses were performed by SWATH mass spectrometry to determine the respective proteomes of planktonic and biofilm cells after exposition to Cu2+ and Ni2+.Quantitative data for 1180 proteins were obtained, corresponding to 46% of the predicted proteome. In planktonic cells, 234 of 1180 proteins showed significant abundance changes after metal ion treatment, of which 47% occurred in Cu2+ and Ni2+ treated samples. In biofilms, significant changes were detected for 52 proteins. Only three proteins changed under both conditions, suggesting metal-specific stress responses in biofilms. Deletion strains were generated to assess the potential role of selected target genes. Strongest effects were observed for Delta OE5245F and Delta OE2816F strains which exhibited increased and decreased biofilm mass after Ni2+ exposure, respectively. Moreover, EPS obviously plays a crucial role in H. salinarum metal ion resistance. Further efforts are required to elucidate the molecular basis and interplay of additional resistance mechanisms.

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Language(s): eng - English
 Dates: 2020-01-17
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.3389/fmicb.2019.03056
 Degree: -

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Title: Frontiers in Microbiology
Source Genre: Journal
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Pages: 15 Volume / Issue: 10 Sequence Number: 3056 Start / End Page: - Identifier: -