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  A function of profilin in force generation during malaria parasite motility independent of actin binding

Moreau, C. A., Quadt, K., Piirainen, H., Kumar, H., Bhargav, S. P., Strauss, L., et al. (2020). A function of profilin in force generation during malaria parasite motility independent of actin binding. Journal of Cell Science, 233775, pp. 1-55. doi:10.1242/jcs.233775.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-A8E3-D Version Permalink: http://hdl.handle.net/21.11116/0000-0006-05AC-3
Genre: Journal Article

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Moreau, Catherine A., Author
Quadt, Katherina1, Author              
Piirainen, Henni, Author
Kumar, Hirdesh, Author
Bhargav, Saligram P., Author
Strauss, Léanne, Author
Tolia, Niraj H., Author
Wade, Rebecca C., Author
Spatz, Joachim P.1, 2, Author              
Kursula, Inari, Author
Frischknecht, Friedrich, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: During transmission of malaria-causing parasites from mosquito to mammal, Plasmodium sporozoites migrate at high speed within the skin to access the bloodstream and infect the liver. This unusual gliding motility is based on retrograde flow of membrane proteins and highly dynamic actin filaments that provide short tracks for a myosin motor. Using laser tweezers and parasite mutants, we previously suggested that actin filaments form macromolecular complexes with plasma-membrane spanning adhesins to generate force during migration. Mutations in the actin-binding region of profilin, a near ubiquitous actin-binding protein, revealed that loss of actin binding also correlates with loss of force production and motility. Here we show that different mutations in profilin, not affecting actin binding in vitro, still generate lower force during Plasmodium sporozoite migration. Lower force generation inversely correlates with increased retrograde flow suggesting that, like in mammalian cells, the slow-down of flow to generate force is the key underlying principle governing Plasmodium gliding motility.

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Language(s): eng - English
 Dates: 2019-10-022020-01-062020-02-07
 Publication Status: Published online
 Pages: 55
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1242/jcs.233775
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Title: Journal of Cell Science
Source Genre: Journal
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Publ. Info: Cambridge, U.K. : Co. of Biologists
Pages: - Volume / Issue: - Sequence Number: 233775 Start / End Page: 1 - 55 Identifier: ISSN: 0021-9533
CoNE: https://pure.mpg.de/cone/journals/resource/954925326678