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  Alginate hydrogels for in vivo bone regeneration : the immune competence of the animal model matters

Garske, D., Schmidt-Bleek, K., Ellinghaus, A., Dienelt, A., Gu, L., Mooney, D. J., et al. (2020). Alginate hydrogels for in vivo bone regeneration: the immune competence of the animal model matters. Tissue Engineering Part A, 26(15-16), 852-862. doi:10.1089/ten.TEA.2019.0310.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-AAC9-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-F3AD-5
Genre: Journal Article

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 Creators:
Garske, Daniela1, Author              
Schmidt-Bleek, Katharina, Author
Ellinghaus, Agnes, Author
Dienelt, Anke, Author
Gu, Luo, Author
Mooney, David J., Author
Duda, Georg, Author
Cipitria, Amaia1, Author              
Affiliations:
1Amaia Cipitria, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2489692              

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Free keywords: alginate; hydrogelbone regeneration; critical-sized bone defect; immune system; animal model
 Abstract: Biomaterials with tunable biophysical properties hold great potential for tissue engineering. The adaptive immune system plays an important role in bone regeneration. Our goal is to investigate the regeneration potential of cell-laden alginate hydrogels depending on the immune status of the animal model. Specifically, the regeneration potential of rat mesenchymal stromal cell (MSC)-laden, void-forming alginate hydrogels, with a stiffness optimized for osteogenic differentiation, is studied in 5 mm critical-sized femoral defects, in both T-cell deficient athymic RNU nude rats and immunocompetent Sprague Dawley rats. Bone volume fraction, bone mineral density and tissue mineral density are higher for athymic RNU nude rats 6 weeks post-surgery. Additionally, these animals show a significantly higher number of total cells and cells with non-lymphocyte morphology at the defect site, while the number of cells with lymphocyte-like morphology is lower. Hydrogel degradation is slower and the remaining alginate fragments are surrounded by a thicker fibrous capsule. Ossification islands originating from alginate residues suggest that encapsulated MSCs differentiate into the osteogenic lineage and initiate the mineralization process. However, this effect is insufficient to fully bridge the bone defect in both animal models. Alginate hydrogels can be used to deliver MSCs and thereby recruit endogenous cells through paracrine signaling, but additional osteogenic stimuli are needed to regenerate critical-sized segmental femoral defects.

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Language(s): eng - English
 Dates: 2020-03-102020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1089/ten.TEA.2019.0310
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Title: Tissue Engineering Part A
  Other : Tissue Engineering / Part A
  Abbreviation : Tissue Eng. A
Source Genre: Journal
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Publ. Info: New Rochelle, NY : Mary Ann Liebert
Pages: - Volume / Issue: 26 (15-16) Sequence Number: - Start / End Page: 852 - 862 Identifier: ISSN: 1938-3341