English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Benchmarking tomographic acquisition schemes for high-resolution structural biology

Turoňová, B., Hagen, W. J. H., Obr, M., Mosalaganti, S., Beugelink, J. W., Zimmerli, C. E., et al. (2020). Benchmarking tomographic acquisition schemes for high-resolution structural biology. Nature Communications, 11: 876. doi:10.1038/s41467-020-14535-2.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Turoňová, Beata1, Author
Hagen, Wim J. H.1, Author
Obr, Martin2, Author
Mosalaganti, Shyamal1, Author
Beugelink, J. Wouter1, 3, Author
Zimmerli, Christian E.1, 4, Author
Kräusslich, Hans-Georg2, Author
Beck, Martin1, 5, Author              
Affiliations:
1European Molecular Biology Laboratory, Structure and Computational Biology Unit, Heidelberg, Germany, ou_persistent22              
2Department of Infectious Diseases, Virology, Universitätsklinikum Heidelberg, Heidelberg, Germany, ou_persistent22              
3Cryo-Electron Microscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Utrecht, The Netherlands, ou_persistent22              
4Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, Heidelberg, Germany, ou_persistent22              
5Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society, ou_3040395              

Content

show
hide
Free keywords: -
 Abstract: Cryo electron tomography with subsequent subtomogram averaging is a powerful technique to structurally analyze macromolecular complexes in their native context. Although close to atomic resolution in principle can be obtained, it is not clear how individual experimental parameters contribute to the attainable resolution. Here, we have used immature HIV-1 lattice as a benchmarking sample to optimize the attainable resolution for subtomogram averaging. We systematically tested various experimental parameters such as the order of projections, different angular increments and the use of the Volta phase plate. We find that although any of the prominently used acquisition schemes is sufficient to obtain subnanometer resolution, dose-symmetric acquisition provides considerably better outcome. We discuss our findings in order to provide guidance for data acquisition. Our data is publicly available and might be used to further develop processing routines.

Details

show
hide
Language(s): eng - English
 Dates: 2019-08-102020-01-162020-02-13
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-020-14535-2
BibTex Citekey: turonova_benchmarking_2020
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 11 Sequence Number: 876 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723