English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Serial protein crystallography in an electron microscope

Bücker, R., Hogan-Lamarre, P., Mehrabi, P., Schulz, E.-C., Bultema, L., Gevorkov, Y., et al. (2020). Serial protein crystallography in an electron microscope. Nature Communications, 11(1): 996. doi:10.1038/s41467-020-14793-0.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-AC09-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-06BC-0
Genre: Journal Article

Files

show Files
hide Files
:
s41467-020-14793-0.pdf (Publisher version), 2MB
Name:
s41467-020-14793-0.pdf
Description:
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
2020
Copyright Info:
© the Author(s)

Locators

show
hide
Description:
-
Locator:
https://dx.doi.org/10.1038/s41467-020-14793-0 (Publisher version)
Description:
-
Description:
Supplementary information

Creators

show
hide
 Creators:
Bücker, R.1, 2, Author              
Hogan-Lamarre, P.1, 2, 3, Author              
Mehrabi, P.1, 2, Author              
Schulz, E.-C.1, 2, Author              
Bultema, L.1, 2, Author              
Gevorkov, Y.4, 5, Author
Brehm, W.4, Author
Yefanov, O.4, Author
Oberthür, D.4, Author
Kassier, G.1, 2, Author              
Miller, R. J. D.1, 2, 3, Author              
Affiliations:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
2Center for Free-Electron Laser Science, ou_persistent22              
3Departments of Chemistry and Physics, University of Toronto, ou_persistent22              
4Center for Free-Electron Laser Science, DESY, ou_persistent22              
5Institute of Vision Systems, Hamburg University of Technology, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Serial X-ray crystallography at free-electron lasers allows to solve biomolecular structures from sub-micron-sized crystals. However, beam time at these facilities is scarce, and involved sample delivery techniques are required. On the other hand, rotation electron diffraction (MicroED) has shown great potential as an alternative means for protein nano-crystallography. Here, we present a method for serial electron diffraction of protein nanocrystals combining the benefits of both approaches. In a scanning transmission electron microscope, crystals randomly dispersed on a sample grid are automatically mapped, and a diffraction pattern at fixed orientation is recorded from each at a high acquisition rate. Dose fractionation ensures minimal radiation damage effects. We demonstrate the method by solving the structure of granulovirus occlusion bodies and lysozyme to resolutions of 1.55 Å and 1.80 Å, respectively. Our method promises to provide rapid structure determination for many classes of materials with minimal sample consumption, using readily available instrumentation.

Details

show
hide
Language(s): eng - English
 Dates: 2019-11-122020-01-272020-02-21
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-020-14793-0
 Degree: -

Event

show

Legal Case

show

Project information

show hide
Project name : We thank Djordje Gitaric for mechanical design work, Fabian Westermeier, David Pennicard, and Heinz Graafsma for adapting the Lambda detector for electron imaging, Anton Barty and Henry Chapman for many helpful discussions and critical reading of the manuscript, Thomas A. White for help with modifying CrystFEL for electron diffraction, and Michiel de Kock for help with image processing. We are indebted to Kay Grünewald and his research group for lending to us their cryo-transfer holder. We gratefully acknowledge the support provided by the Max Planck Society, Deutsches Elektronen-Synchrotron (DESY), the excellence cluster “The Hamburg Center for Ultrafast Imaging” of the Deutsche Forschungsgemeinschaft (EXC 1074 project ID 194651731), the European Research Council project “Attosecond X-ray Science: Imaging and Spectroscopy” (Award/Contract Number ERC-2013-SyG 609920), and the Joachim Herz Foundation (Biomedical Physics of Infection). P.H. acknowledges support by the Natural Sciences and Engineering Research Council of Canada. P.M. was supported by the Alexander von Humboldt-Stiftung for postdoctoral researchers.
Grant ID : -
Funding program : -
Funding organization : -

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 11 (1) Sequence Number: 996 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723