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  Serial protein crystallography in an electron microscope

Bücker, R., Hogan-Lamarre, P., Mehrabi, P., Schulz, E.-C., Bultema, L., Gevorkov, Y., Brehm, W., Yefanov, O., Oberthür, D., Kassier, G., & Miller, R. J. D. (2020). Serial protein crystallography in an electron microscope. Nature Communications, 11(1):. doi:10.1038/s41467-020-14793-0.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0005-AC09-0 版のパーマリンク: https://hdl.handle.net/21.11116/0000-0006-06BC-0
資料種別: 学術論文

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s41467-020-14793-0.pdf (出版社版), 2MB
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https://hdl.handle.net/21.11116/0000-0005-B1AF-E
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s41467-020-14793-0.pdf
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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
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著作権日付:
2020
著作権情報:
© the Author(s)
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https://creativecommons.org/licenses/by/4.0/

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URL:
https://dx.doi.org/doi.org/10.1101/682575 (プレプリント)
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https://dx.doi.org/10.1038/s41467-020-14793-0 (出版社版)
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https://www.nature.com/articles/s41467-020-14793-0#Sec13 (付録資料)
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Supplementary information
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作成者

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 作成者:
Bücker, R.1, 2, 著者           
Hogan-Lamarre, P.1, 2, 3, 著者           
Mehrabi, P.1, 2, 著者           
Schulz, E.-C.1, 2, 著者           
Bultema, L.1, 2, 著者           
Gevorkov, Y.4, 5, 著者
Brehm, W.4, 著者
Yefanov, O.4, 著者
Oberthür, D.4, 著者
Kassier, G.1, 2, 著者           
Miller, R. J. D.1, 2, 3, 著者           
所属:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
2Center for Free-Electron Laser Science, ou_persistent22              
3Departments of Chemistry and Physics, University of Toronto, ou_persistent22              
4Center for Free-Electron Laser Science, DESY, ou_persistent22              
5Institute of Vision Systems, Hamburg University of Technology, ou_persistent22              

内容説明

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 要旨: Serial X-ray crystallography at free-electron lasers allows to solve biomolecular structures from sub-micron-sized crystals. However, beam time at these facilities is scarce, and involved sample delivery techniques are required. On the other hand, rotation electron diffraction (MicroED) has shown great potential as an alternative means for protein nano-crystallography. Here, we present a method for serial electron diffraction of protein nanocrystals combining the benefits of both approaches. In a scanning transmission electron microscope, crystals randomly dispersed on a sample grid are automatically mapped, and a diffraction pattern at fixed orientation is recorded from each at a high acquisition rate. Dose fractionation ensures minimal radiation damage effects. We demonstrate the method by solving the structure of granulovirus occlusion bodies and lysozyme to resolutions of 1.55 Å and 1.80 Å, respectively. Our method promises to provide rapid structure determination for many classes of materials with minimal sample consumption, using readily available instrumentation.

資料詳細

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言語: eng - English
 日付: 2019-11-122020-01-272020-02-21
 出版の状態: オンラインで出版済み
 ページ: -
 出版情報: -
 目次: -
 査読: 査読あり
 識別子（DOI, ISBNなど）: DOI: 10.1038/s41467-020-14793-0
 学位: -

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Project information

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Project name : We thank Djordje Gitaric for mechanical design work, Fabian Westermeier, David Pennicard, and Heinz Graafsma for adapting the Lambda detector for electron imaging, Anton Barty and Henry Chapman for many helpful discussions and critical reading of the manuscript, Thomas A. White for help with modifying CrystFEL for electron diffraction, and Michiel de Kock for help with image processing. We are indebted to Kay Grünewald and his research group for lending to us their cryo-transfer holder. We gratefully acknowledge the support provided by the Max Planck Society, Deutsches Elektronen-Synchrotron (DESY), the excellence cluster “The Hamburg Center for Ultrafast Imaging” of the Deutsche Forschungsgemeinschaft (EXC 1074 project ID 194651731), the European Research Council project “Attosecond X-ray Science: Imaging and Spectroscopy” (Award/Contract Number ERC-2013-SyG 609920), and the Joachim Herz Foundation (Biomedical Physics of Infection). P.H. acknowledges support by the Natural Sciences and Engineering Research Council of Canada. P.M. was supported by the Alexander von Humboldt-Stiftung for postdoctoral researchers.
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出版物 1

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出版物名: Nature Communications
  省略形 : Nat. Commun.
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: London : Nature Publishing Group
ページ: - 巻号: 11 (1) 通巻号: 996 開始・終了ページ: - 識別子（ISBN, ISSN, DOIなど）: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723