English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Social cooperativity of bacteria during eversible surface attachment in young biofilms: A quantitative comparison of Pseudomonas aeruginosa PA14 and PAO1

Lee, C. K., Vachier, J., de Anda, J., Zhao, K., Baker, A. E., Bennett, R. R., et al. (2020). Social cooperativity of bacteria during eversible surface attachment in young biofilms: A quantitative comparison of Pseudomonas aeruginosa PA14 and PAO1. mBio, 11(1): e02644-19. doi:10.1128/mBio.02644-19.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-B8B8-C Version Permalink: http://hdl.handle.net/21.11116/0000-0005-BD87-E
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Lee, Calvin K., Author
Vachier, Jérémy1, Author              
de Anda, Jaime, Author
Zhao, Kun, Author
Baker, Amy E., Author
Bennett, Rachel R., Author
Armbruster, Catherine R., Author
Lewis, Kimberley A., Author
Tarnopol, Rebecca L., Author
Lomba, Charles J., Author
Hogan, Deborah A., Author
Parsek, Matthew R., Author
O'Toole, George A., Author
Golestanian, Ramin2, Author              
Wong, Gerard C. L., Author
Affiliations:
1Group Non-equilibrium soft matter, Department of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063308              
2Department of Living Matter Physics, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2570692              

Content

show
hide
Free keywords: -
 Abstract: What are bacteria doing during “reversible attachment,” the period of transient surface attachment when they initially engage a surface, besides attaching themselves to the surface? Can an attaching cell help any other cell attach? If so, does it help all cells or employ a more selective strategy to help either nearby cells (spatial neighbors) or its progeny (temporal neighbors)? Using community tracking methods at the single-cell resolution, we suggest answers to these questions based on how reversible attachment progresses during surface sensing for Pseudomonas aeruginosa strains PAO1 and PA14. Although PAO1 and PA14 exhibit similar trends of surface cell population increase, they show unanticipated differences when cells are considered at the lineage level and interpreted using the quantitative framework of an exactly solvable stochastic model. Reversible attachment comprises two regimes of behavior, processive and nonprocessive, corresponding to whether cells of the lineage stay on the surface long enough to divide, or not, before detaching. Stark differences between PAO1 and PA14 in the processive regime of reversible attachment suggest the existence of two surface colonization strategies. PAO1 lineages commit quickly to a surface compared to PA14 lineages, with early c-di-GMP-mediated exopolysaccharide (EPS) production that can facilitate the attachment of neighbors. PA14 lineages modulate their motility via cyclic AMP (cAMP) and retain memory of the surface so that their progeny are primed for improved subsequent surface attachment. Based on the findings of previous studies, we propose that the differences between PAO1 and PA14 are potentially rooted in downstream differences between Wsp-based and Pil-Chp-based surface-sensing systems, respectively.

Details

show
hide
Language(s): eng - English
 Dates: 2020-02-252020
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1128/mBio.02644-19
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: mBio
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, DC : American Society for Microbiology
Pages: 21 Volume / Issue: 11 (1) Sequence Number: e02644-19 Start / End Page: - Identifier: ISSN: 2150-7511
CoNE: https://pure.mpg.de/cone/journals/resource/2150-7511