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  Chemogenetic control of nanobodies

Farrants, H., Tarnawski, M., Müller, T. G., Otsuka, S., Hiblot, J., Koch, B., et al. (2020). Chemogenetic control of nanobodies. Nature methods, 17, 279-282. doi:10.1038/s41592-020-0746-7.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-BA70-B Version Permalink: http://hdl.handle.net/21.11116/0000-0006-095A-C
Genre: Journal Article

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 Creators:
Farrants, Helen1, Author              
Tarnawski, Miroslaw, Author
Müller, Thorsten G., Author
Otsuka, Shotaro, Author
Hiblot, Julien1, Author              
Koch, Birgit1, Author              
Kueblbeck, Moritz, Author
Kräusslich, Hans-Georg, Author
Ellenberg, Jan, Author
Johnsson, Kai1, Author              
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Abstract: We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.

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Language(s): eng - English
 Dates: 2020-02-172020-03-01
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-020-0746-7
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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 17 Sequence Number: - Start / End Page: 279 - 282 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556