English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-throughput platform for optoacoustic probing of genetically encoded calcium ion indicators

Hofmann, U. A. T., Fabritius, A., Rebling, J., Estrada, H., Dean-Ben, X. L., Griesbeck, O., et al. (2019). High-throughput platform for optoacoustic probing of genetically encoded calcium ion indicators. iScience, 22, 400-408. doi:10.1016/j.isci.2019.11.034.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
1-s2.0-S2589004219304845-main.pdf (Publisher version), 3MB
Name:
1-s2.0-S2589004219304845-main.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
open access
:
1-s2.0-S2589004219304845-mmc1.pdf (Supplementary material), 333KB
Name:
1-s2.0-S2589004219304845-mmc1.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Hofmann, Urs A. T., Author
Fabritius, Arne1, Author           
Rebling, Johannes, Author
Estrada, Hector, Author
Dean-Ben, X. Luis, Author
Griesbeck, Oliver1, Author           
Razansky, Daniel, Author
Affiliations:
1Research Group: Tools for Bio-Imaging / Griesbeck, MPI of Neurobiology, Max Planck Society, ou_1113560              

Content

show
hide
Free keywords: PHOTOACOUSTIC MICROSCOPY; NEURONAL-ACTIVITY; PROTEINS
 Abstract: Functional optoacoustic (OA) imaging assisted with genetically encoded calcium ion indicators (GECIs) holds promise for imaging large-scale neuronal activity at depths and spatiotemporal resolutions not attainable with existing optical microscopic techniques. However, currently available GECIs optimized for fluorescence (FL) imaging lack sufficient contrast for OA imaging and respond at wavelengths having limited penetration into the mammalian brain. Here we present an imaging platform capable of rapid assessment and cross-validation between OA and FL responses of sensor proteins expressed in Escherichia coli colonies. The screening system features optimized pulsed light excitation combined with ultrasensitive ultrasound detection to mitigate photobleaching while further allowing the dynamic characterization of calcium ion responses with millisecond precision. Targeted probing of up to six individual colonies per second in both calcium-loaded and calcium-unloaded states was possible with the system. The new platform greatly facilitates optimization of absorption-based labels, thus setting the stage for directed evolution of OA GECIs.

Details

show
hide
Language(s): eng - English
 Dates: 2019-12-20
 Publication Status: Published in print
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show hide
Project name : National Institute of Health grant R21-EY028365 (O.G. and D.R.)
Grant ID : R21-EY028365
Funding program : -
Funding organization : -

Source 1

show
hide
Title: iScience
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis : Elsevier
Pages: - Volume / Issue: 22 Sequence Number: - Start / End Page: 400 - 408 Identifier: ISSN: 2589-0042
CoNE: https://pure.mpg.de/cone/journals/resource/2589-0042