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  High-resolution 3D light microscopy with STED and RESOLFT

Sahl, S. J., & Hell, S. W. (2019). High-resolution 3D light microscopy with STED and RESOLFT. In J. F. Bille (Ed.), High resolution imaging in microscopy and ophthalmology (pp. 3-32). Cham: Springer. doi:10.1007/978-3-030-16638-0_1.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-BE27-A Version Permalink: http://hdl.handle.net/21.11116/0000-0005-BE29-8
Genre: Book Chapter

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 Creators:
Sahl, S. J.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Free keywords: Fluorescence imaging ; Superresolution ; Nanoscopy ; Molecular states : Diffraction-unlimited resolution ; Far-field optics ; Neuroscience ; Cell biology ; Stimulated emission ; Switchable proteins
 Abstract: This chapter discusses the simple yet powerful ideas which have allowed to break the diffraction resolution limit of lens-based optical microscopy. The basic principles and standard implementations of STED (stimulated emission depletion) and RESOLFT (reversible saturable/switchable optical linear (fluorescence) transitions) microscopy are introduced, followed by selected highlights of recent advances, including MINFLUX (minimal photon fluxes) nanoscopy with molecule-size (~1 nm) resolution.

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Language(s): eng - English
 Dates: 2019-08-142019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/978-3-030-16638-0_1
 Degree: -

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Title: High resolution imaging in microscopy and ophthalmology
Source Genre: Book
 Creator(s):
Bille, Josef F., Editor
Affiliations:
-
Publ. Info: Cham : Springer
Pages: XX, 407 Volume / Issue: - Sequence Number: - Start / End Page: 3 - 32 Identifier: ISBN: 978-3-030-16637-3
ISBN: 978-3-030-16638-0