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  Determination of methyl order parameters using solid state NMR under off magic angle spinning.

Xue, K., Mamone, S., Koch, B., Sarkar, R., & Reif, B. (2019). Determination of methyl order parameters using solid state NMR under off magic angle spinning. Journal of Biomolecular NMR, (8-9), 471-475. doi:10.1007/s10858-019-00253-5.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-BF4E-E Version Permalink: http://hdl.handle.net/21.11116/0000-0005-BF54-6
Genre: Journal Article

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3196179.pdf (Publisher version), 945KB
 
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 Creators:
Xue, K., Author
Mamone, S.1, Author              
Koch, B., Author
Sarkar, R., Author
Reif, B., Author
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1Research Group of NMR Signal Enhancement, MPI for Biophysical Chemistry, Max Planck Society, ou_2396691              

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Free keywords: Solid-state NMR; Off magic angle spinning; Spin Echo; Microcrystalline proteins; Perdeuteration
 Abstract: Quantification of dipolar couplings in biological solids is important for the understanding of dynamic processes. Under Magic Angle Spinning (MAS), order parameters are normally obtained by recoupling of anisotropic interactions involving the application of radio frequency pulses. We have recently shown that amide backbone order parameters can be estimated accurately in a spin-echo experiment in case the rotor spinning angle is slightly mis-calibrated. In this work, we apply this method to determine methyl order parameters in a deuterated sample of the SH3 domain of chicken a-spectrin in which the methyl containing side chains valine and leucine are selectively protonated.

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Language(s): eng - English
 Dates: 2019-08-122019-09
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s10858-019-00253-5
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Title: Journal of Biomolecular NMR
Source Genre: Journal
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Pages: - Volume / Issue: (8-9) Sequence Number: - Start / End Page: 471 - 475 Identifier: -