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  The HARE chip for efficient time-resolved serial synchrotron crystallography

Mehrabi, P., Müller-Werkmeister, H., Leimkohl, J.-P., Schikora, H., Ninkovic, J., Krivokuca, S., et al. (2020). The HARE chip for efficient time-resolved serial synchrotron crystallography. Journal of Synchrotron Radiation; Wiley-Blackwell on behalf of the IUCr, 27(2), 360-370. doi:10.1107/S1600577520000685.

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suppl.zip (Supplementary material), 6MB
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Figure S1: SEM Images of the HARE chip at different magnifications (pdf) | Supporting material 1 to 10 (CAD, 3D printer files, etc.) to reproduce the HARE chip and the accessory material (zip)
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This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
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 Creators:
Mehrabi, P.1, Author           
Müller-Werkmeister, H.1, 2, Author           
Leimkohl, J.-P.3, Author           
Schikora, H.3, Author           
Ninkovic, J.4, Author
Krivokuca, S.4, Author
Andriček, L.4, Author
Epp, S. W.1, Author           
Sherrell, D.5, Author
Owen, R. L.5, Author
Pearson, A. R.6, Author
Tellkamp, F.3, Author           
Schulz, E.-C.1, Author           
Miller, R. J. D.1, 6, 7, Author           
Affiliations:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
2Institute of Chemistry – Physical Chemistry, University of Potsdam, ou_persistent22              
3Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              
4Halbleiterlabor der Max-Planck-Gesellschaft, ou_persistent22              
5Diamond Light Source, Harwell Science and Innovation Campus, ou_persistent22              
6Department of Physics, Universität Hamburg, ou_persistent22              
7Departments of Chemistry and Physics, University of Toronto, ou_persistent22              

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Free keywords: data collection; sample delivery; fixed-target serial synchrotron crystallography; time-resolved crystallography; HARE; LAMA
 Abstract: Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.

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Language(s): eng - English
 Dates: 2019-11-192020-01-202020-02-272020-03
 Publication Status: Issued
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1107/S1600577520000685
 Degree: -

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Project name : -
Grant ID : 623994
Funding program : Funding Programme 7 (FP7)
Funding organization : European Commission (EC)
Project name : Support was provided by the Max Planck Society and by the Cluster of Excellence `The Hamburg Centre for Ultrafast Imaging' of the Deutsche Forschungsgemeinschaft (EXC 1074) project ID 194651731 (RJDM), the People Program (Marie Curie Actions) of the European Union's Seventh Framework Program (FP7/2007–2013) under REA grant agreement No. 623994 (HMW) and the Joachim Herz Foundation (Biomedical Physics of Infection). PM was the recipient of an Alexander von Humboldt-Stiftung postdoctoral research award. AP received funding for development and the construction of the T-REXX SSX endstation from the BMBF (`Verbundforschung', 05K16GU1 and 05K19GU1).
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Title: Journal of Synchrotron Radiation; Wiley-Blackwell on behalf of the IUCr
Source Genre: Journal
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Pages: 11 Volume / Issue: 27 (2) Sequence Number: - Start / End Page: 360 - 370 Identifier: -