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  Urine E-cadherin: A Marker for early detection of kidney injury in diabetic patients.

Koziolek, M., Mueller, G. A., Dihazi, G. H., Jung, K., Altubar, C., Wallbach, M., et al. (2020). Urine E-cadherin: A Marker for early detection of kidney injury in diabetic patients. Journal of Clinical Medicine, 9(3): 639. doi:10.3390/jcm9030639.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-C4B6-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-CFFC-6
Genre: Journal Article

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 Creators:
Koziolek, M., Author
Mueller, G. A., Author
Dihazi, G. H., Author
Jung, K., Author
Altubar, C., Author
Wallbach, M., Author
Markovic, I., Author
Raddatz, D., Author
Jahn, O., Author
Karaköse, H., Author
Lenz, C.1, Author              
Urlaub, H.1, Author              
Dihazi, A., Author
El Meziane, A., Author
Dihazi, H., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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Free keywords: Diabetic nephropathy; E-cadherin; early biomarker; early diagnosis/prognosis
 Abstract: Diabetic nephropathy (DN) is the main reason for end-stage renal disease. Microalbuminuria as the non-invasive available diagnosis marker lacks specificity and gives high false positive rates. To identify and validate biomarkers for DN, we used in the present study urine samples from four patient groups: diabetes without nephropathy, diabetes with microalbuminuria, diabetes with macroalbuminuria and proteinuria without diabetes. For the longitudinal validation, we recruited 563 diabetic patients and collected 1363 urine samples with the clinical data during a follow-up of 6 years. Comparative urinary proteomics identified four proteins Apolipoprotein A-I (APOA1), Beta-2-microglobulin (B2M), E-cadherin (CDH1) and Lithostathine-1-alpha (REG1A), which differentiated with high statistical strength (p < 0.05) between DN patients and the other groups. Label-free mass spectrometric quantification of the candidates confirmed the discriminatory value of E-cadherin and Lithostathine-1-alpha (p < 0.05). Immunological validation highlighted E-cadherin as the only marker able to differentiate significantly between the different DN stages with an area under the curve (AUC) of 0.85 (95%-CI: [0.72, 0.97]). The analysis of the samples from the longitudinal study confirmed the prognostic value of E-cadherin, the critical increase in urinary E-cadherin level was measured 20 ± 12.5 months before the onset of microalbuminuria and correlated significantly (p < 0.05) with the glomerular filtration rate measured by estimated glomerular filtration rate (eGFR).

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Language(s): eng - English
 Dates: 2020-02-27
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3390/jcm9030639
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Title: Journal of Clinical Medicine
Source Genre: Journal
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Pages: 21 Volume / Issue: 9 (3) Sequence Number: 639 Start / End Page: - Identifier: -