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  Mitochondria supply membranes for autophagosome biogenesis during starvation

Hailey, D. W., Rambold, A., Satpute-Krishnan, P., Mitra, K., Sougrat, R., Kim, P. K., et al. (2010). Mitochondria supply membranes for autophagosome biogenesis during starvation. Cell, 141, 656-667. doi:org/10.1016/j.cell.2010.04.009.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-C52A-E Version Permalink: http://hdl.handle.net/21.11116/0000-0005-C52B-D
Genre: Journal Article

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Hailey, Dale W.1, Author
Rambold, Angelika2, Author              
Satpute-Krishnan, Prasanna1, Author
Mitra, Kasturi1, Author
Sougrat, Rachid1, Author
Kim, Peter K.1, Author
Lippincott-Schwartz, Jennifer1, Author
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1External Organizations, ou_persistent22              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243650              

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 Abstract: Starvation-induced autophagosomes engulf cytosol and/or organelles and deliver them to lysosomes for degradation, thereby resupplying depleted nutrients. Despite advances in understanding the molecular basis of this process, the membrane origin of autophagosomes remains unclear. Here, we demonstrate that, in starved cells, the outer membrane of mitochondria participates in autophagosome biogenesis. The early autophagosomal marker, Atg5, transiently localizes to punctae on mitochondria, followed by the late autophagosomal marker, LC3. The tail-anchor of an outer mitochondrial membrane protein also labels autophagosomes and is sufficient to deliver another outer mitochondrial membrane protein, Fis1, to autophagosomes. The fluorescent lipid NBD-PS (converted to NBD-phosphotidylethanolamine in mitochondria) transfers from mitochondria to autophagosomes. Photobleaching reveals membranes of mitochondria and autophagosomes are transiently shared. Disruption of mitochondria/ER connections by mitofusin2 depletion dramatically impairs starvation-induced autophagy. Mitochondria thus play a central role in starvation-induced autophagy, contributing membrane to autophagosomes.

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Language(s): eng - English
 Dates: 2010
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: org/10.1016/j.cell.2010.04.009
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Title: Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 141 Sequence Number: - Start / End Page: 656 - 667 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183