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  Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regulators of Adult Neurogenesis

Kjell, J., Fischer-Sternjak, J., Thompson, A. J., Friess, C., Sticco, M. J., Salinas, F., et al. (2020). Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regulators of Adult Neurogenesis. CELL STEM CELL, 26(2), 277-293.e8. doi:10.1016/j.stem.2020.01.002.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-C6B0-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-C6B1-3
Genre: Journal Article

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 Creators:
Kjell, Jacob1, Author
Fischer-Sternjak, Judith1, Author
Thompson, Amelia J.1, Author
Friess, Christian1, Author
Sticco, Matthew J.1, Author
Salinas, Favio2, 3, Author              
Cox, Jürgen2, 3, Author              
Martinelli, David C.1, Author
Ninkovic, Jovica1, Author
Franze, Kristian1, Author
Schiller, Herbert B.2, Author              
Götz, Magdalena1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
3Cox, Jürgen / Computational Systems Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_2063284              

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Free keywords: MOUSE SUBEPENDYMAL ZONE; EXTRACELLULAR-MATRIX; PROGENITOR CELLS; NEURONAL DIFFERENTIATION; TENASCIN-C; RAT-BRAIN; MIGRATION; LINEAGE; TRANSPLANTATION; PROLIFERATION
 Abstract: The mammalian brain contains few niches for neural stem cells (NSCs) capable of generating new neurons, whereas other regions are primarily gliogenic. Here we leverage the spatial separation of the sub-ependymal zone NSC niche and the olfactory bulb, the region to which newly generated neurons from the sub-ependymal zone migrate and integrate, and present a comprehensive proteomic characterization of these regions in comparison to the cerebral cortex, which is not conducive to neurogenesis and integration of new neurons. We find differing compositions of regulatory extracellular matrix (ECM) components in the neurogenic niche. We further show that quiescent NSCs are the main source of their local ECM, including the multi-functional enzyme transglutaminase 2, which we show is crucial for neurogenesis. Atomic force microscopy corroborated indications from the proteomic analyses that neurogenic niches are significantly stiffer than non-neurogenic parenchyma. Together these findings provide a powerful resource for unraveling unique compositions of neurogenic niches.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: 25
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: CELL STEM CELL
Source Genre: Journal
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Publ. Info: 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 26 (2) Sequence Number: - Start / End Page: 277 - 293.e8 Identifier: ISSN: 1934-5909