English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Singlet-filtered NMR spectroscopy.

Mamone, S., Rezaei-Ghaleh, N., Opazo, F., Griesinger, C., & Glöggler, S. (2020). Singlet-filtered NMR spectroscopy. Science Advances, 6(8): eaaz1955. doi:10.1126/sciadv.aaz1955.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-C90C-C Version Permalink: http://hdl.handle.net/21.11116/0000-0005-C92D-7
Genre: Journal Article

Files

show Files
hide Files
:
3212427.pdf (Publisher version), 2MB
Name:
3212427.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
3212427_Suppl.DC1 (Supplementary material), 60KB
Name:
3212427_Suppl.DC1
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/xhtml+xml / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Mamone, S.1, Author              
Rezaei-Ghaleh, N.2, Author              
Opazo, F., Author
Griesinger, C.3, Author              
Glöggler, S.1, Author              
Affiliations:
1Research Group of NMR Signal Enhancement, MPI for Biophysical Chemistry, Max Planck Society, ou_2396691              
2Research Group of Protein Strcture Determination using NMR, MPI for biophysical chemistry, Max Planck Society, ou_578571              
3Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

Content

show
hide
Free keywords: -
 Abstract: Selectively studying parts of proteins and metabolites in tissue with nuclear magnetic resonance promises new insights into molecular structures or diagnostic approaches. Nuclear spin singlet states allow the selection of signals from chemical moieties of interest in proteins or metabolites while suppressing background signal. This selection process is based on the electron-mediated coupling between two nuclear spins and their difference in resonance frequency. We introduce a generalized and versatile pulsed NMR experiment that allows populating singlet states on a broad scale of coupling patterns. This approach allowed us to filter signals from proton pairs in the Alzheimer's disease-related b-amyloid 40 peptide and in metabolites in brain matter. In particular, for glutamine/glutamate, we have discovered a long-lived state in tissue without the typically required singlet sustaining by radiofrequency irradiation. We believe that these findings will open up new opportunities to study metabolites with a view on future in vivo applications.

Details

show
hide
Language(s): eng - English
 Dates: 2020-02-21
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1126/sciadv.aaz1955
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Science Advances
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 7 Volume / Issue: 6 (8) Sequence Number: eaaz1955 Start / End Page: - Identifier: -