English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  ADAPT Identifies an ESCRT Complex Composition That Discriminates VCaP From LNCaP Prostate Cancer Cell Exosomes

Hornung, T., O'Neill, H. A., Logie, S. C., Fowler, K. M., Duncan, J. E., Rosenow, M., et al. (2020). ADAPT Identifies an ESCRT Complex Composition That Discriminates VCaP From LNCaP Prostate Cancer Cell Exosomes. Nucleic Acids Research (London), 48(8), 4013-4027. doi:10.1093/nar/gkaa034.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
gkaa034.pdf (Any fulltext), 2MB
Name:
gkaa034.pdf
Description:
Open Access
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Hornung, Tassilo1, Author
O'Neill, Heather A.1, Author
Logie, Stephen C.1, Author
Fowler, Kimberly M.1, Author
Duncan, Janet E.1, Author
Rosenow, Matthew1, Author
Bondre, Aniket S.1, Author
Tinder, Teresa1, Author
Maher, Varun1, Author
Zarkovic, Jelena1, Author
Zenyu, Zhong1, Author
Richards, Melissa N.1, Author
Wei, Xixi1, Author
Miglarese, Mark R.1, Author
Mayer, Günter1, Author
Famulok, Michael2, Author              
Spetzler, David1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Max Planck Fellow Chemical Biology, Center of Advanced European Studies and Research (caesar), Max Planck Society, ou_2173681              

Content

show
hide
Free keywords: Chemical Biology and Nucleic Acid Chemistry
 Abstract: Libraries of single-stranded oligodeoxynucleotides (ssODNs) can be enriched for sequences that specifically bind molecules on naïve complex biological samples like cells or tissues. Depending on the enrichment strategy, the ssODNs can identify molecules specifically associated with a defined biological condition, for example a pathological phenotype, and thus are potentially useful for biomarker discovery. We performed ADAPT, a variant of SELEX, on exosomes secreted by VCaP prostate cancer cells. A library of ∼1011 ssODNs was enriched for those that bind to VCaP exosomes and discriminate them from exosomes derived from LNCaP prostate cancer cells. Next-generation sequencing (NGS) identified the best discriminating ssODNs, nine of which were resynthesized and their discriminatory ability confirmed by qPCR. Affinity purification with one of the sequences (Sequence 7) combined with LC-MS/MS identified its molecular target complex, whereof most proteins are part of or associated with the multiprotein ESCRT complex participating in exosome biogenesis. Within this complex, YBX1 was identified as the directly-bound target protein. ADAPT thus is able to differentiate exosomes from cancer cell subtypes from the same lineage. The composition of ESCRT complexes in exosomes from VCaP versus LNCaP cells might constitute a discriminatory element between these prostate cancer subtypes.

Details

show
hide
Language(s): eng - English
 Dates: 2020-01-282020-05-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1093/nar/gkaa034
PII: gkaa034
PMID: 31989173
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nucleic Acids Research (London)
  Abbreviation : Nucleic Acids Res
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford : Oxford University Press
Pages: - Volume / Issue: 48 (8) Sequence Number: - Start / End Page: 4013 - 4027 Identifier: ISSN: 0305-1048
CoNE: https://pure.mpg.de/cone/journals/resource/110992357379342