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  Chromatin immunoprecipitation in adult zebrafish red cells

Trompouki, E., Bowman, T. V., DiBiase, A., Zhou, Y., & Zon, L. I. (2011). Chromatin immunoprecipitation in adult zebrafish red cells. Methods in Cell Biology, 104, 341-352. doi:10.1016/B978-0-12-374814-0.00019-7.

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Genre: Journal Article

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 Creators:
Trompouki, Eirini1, Author              
Bowman, Teresa V.2, Author
DiBiase, Anthony2, Author
Zhou, Yi2, Author
Zon, Leonard I.2, Author
Affiliations:
1Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              
2External Organizations, ou_persistent22              

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 Abstract: Zebrafish has been used for many years as a model to study development and disease. The ability of zebrafish to produce thousand of embryos in a synchronous manner has made zebrafish an invaluable tool for genetic and chemical screens. Since its emergence as an important model organism the molecular tools for studying zebrafish have been limited. In this chapter, we describe a simple method to identify DNA binding sites and chromatin architecture in erythrocytes from adult zebrafish using chromatin immunoprecipitation coupled with next generation sequencing. This technique has been used extensively and successfully in other systems and it will be a useful tool for studying epigenetics in zebrafish.

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Language(s): eng - English
 Dates: 2011
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Methods in Cell Biology
  Other : Methods Cell Biol.
Source Genre: Journal
 Creator(s):
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Publ. Info: San Diego [etc.] : Academic Press
Pages: - Volume / Issue: 104 Sequence Number: - Start / End Page: 341 - 352 Identifier: ISSN: 0091-679X
CoNE: https://pure.mpg.de/cone/journals/resource/991042724273554