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  Cryo-electron microscopy of vitrified insect flight muscle

McDowall, A. W., Hofmann, W., Lepault, J., Adrian, M., & Dubochet, J. (1984). Cryo-electron microscopy of vitrified insect flight muscle. Journal of Molecular Biology (London), 178(1), 105-111. doi:10.1016/0022-2836(84)90233-X.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0005-DAC1-B Version Permalink: https://hdl.handle.net/21.11116/0000-0005-DAC2-A
Genre: Journal Article

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JMolBiol_178_1984_105.pdf (Any fulltext), 4MB
 
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McDowall , A. W., Author
Hofmann, Waltraut1, Author           
Lepault, J., Author
Adrian, M., Author
Dubochet, J., Author
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1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: Frozen hydrated sections of vitrified unstained insect flight muscle in the rigor and relaxed states were studied by cryo-electron microscopy. High resolution images with good contrast were obtained. As judged from direct images and from the comparison of optical diffractograms with X-ray diffraction patterns obtained from glycerinated muscle fibres, the vitrified specimen is well preserved. Preparation artifacts encountered in conventional plastic sections are avoided. The preservation of the relaxed structure also seems better than in freeze-etched replicas as the 14.5 nm repeat of crossbridges on thick filaments is easily visible.

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Language(s): eng - English
 Dates: 1984-01-131984-04-121984-09-05
 Publication Status: Issued
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0022-2836(84)90233-X
URI: https://www.ncbi.nlm.nih.gov/pubmed/6481807
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Title: Journal of Molecular Biology (London)
  Other : J Mol Biol
Source Genre: Journal
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Publ. Info: London : Academic Press
Pages: - Volume / Issue: 178 (1) Sequence Number: - Start / End Page: 105 - 111 Identifier: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/954922646042