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  Considerations when investigating IncRNA function in vivo

Bassett, A. R., Akhtar, A., Barlow, D. P., Bird, A. P., Brockdorff, N., Duboule, D., et al. (2014). Considerations when investigating IncRNA function in vivo. eLife, 3: e03058. doi:10.7554/eLife.03058.001.

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Bassett et al..pdf (Publisher version), 781KB
 
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https://elifesciences.org/articles/03058 (Publisher version)
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 Creators:
Bassett, Andrew R 1, Author
Akhtar, Asifa2, Author              
Barlow, Denise P1, Author
Bird, Adrian P1, Author
Brockdorff, Neil1, Author
Duboule, Denis1, Author
Ephrussi, Anne1, Author
Ferguson-Smith, Anne C1, Author
Gingeras, Thomas R1, Author
Haerty, Wilfried1, Author
Higgs, Douglas R1, Author
Miska, Eric A1, Author
Ponting, Chris P1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Department of Chromatin Regulation, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243643              

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 Abstract: Although a small number of the vast array of animal long non-coding RNAs (lncRNAs) have known effects on cellular processes examined in vitro, the extent of their contributions to normal cell processes throughout development, differentiation and disease for the most part remains less clear. Phenotypes arising from deletion of an entire genomic locus cannot be unequivocally attributed either to the loss of the lncRNA per se or to the associated loss of other overlapping DNA regulatory elements. The distinction between cis- or trans-effects is also often problematic. We discuss the advantages and challenges associated with the current techniques for studying the in vivo function of lncRNAs in the light of different models of lncRNA molecular mechanism, and reflect on the design of experiments to mutate lncRNA loci. These considerations should assist in the further investigation of these transcriptional products of the genome.

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Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.7554/eLife.03058.001
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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 3 Sequence Number: e03058 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X