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  On-chip inverted emulsion method for fast giant vesicle production, handling, and analysis

Yandrapalli, N., Seemann, T., & Robinson, T. (2020). On-chip inverted emulsion method for fast giant vesicle production, handling, and analysis. Micromachines, 11(3): 285. doi:10.3390/mi11030285.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-E0DD-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-E0DE-4
Genre: Journal Article

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 Creators:
Yandrapalli, Naresh1, Author              
Seemann, Tina2, Author              
Robinson, Tom1, Author              
Affiliations:
1Tom Robinson, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2288691              
2Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863289              

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Free keywords: lab-on-chip; inverted emulsion method; microfluidics; giant vesicles; giant unilamellar vesicles (GUVs); bottom-up synthetic biology
 Abstract: Liposomes and giant unilamellar vesicles (GUVs) in particular are excellent compartments for constructing artificial cells. Traditionally, their use requires bench-top vesicle growth, followed by experimentation under a microscope. Such steps are time-consuming and can lead to loss of vesicles when they are transferred to an observation chamber. To overcome these issues, we present an integrated microfluidic chip which combines GUV formation, trapping, and multiple separate experiments in the same device. First, we optimized the buffer conditions to maximize both the yield and the subsequent trapping of the vesicles in micro-posts. Captured GUVs were monodisperse with specific size of 18 ± 4 µm in diameter. Next, we introduce a two-layer design with integrated valves which allows fast solution exchange in less than 20 s and on separate sub-populations of the trapped vesicles. We demonstrate that multiple experiments can be performed in a single chip with both membrane transport and permeabilization assays. In conclusion, we have developed a versatile all-in-one microfluidic chip with capabilities to produce and perform multiple experiments on a single batch of vesicles using low sample volumes. We expect this device will be highly advantageous for bottom-up synthetic biology where rapid encapsulation and visualization is required for enzymatic reactions.

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Language(s): eng - English
 Dates: 2020-03-102020
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.3390/mi11030285
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Title: Micromachines
Source Genre: Journal
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Publ. Info: Basel : MDPI
Pages: - Volume / Issue: 11 (3) Sequence Number: 285 Start / End Page: - Identifier: ISSN: 2072-666X