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  Megahertz serial crystallography

Wiedorn, M. O., et, a., & Paulraj, L. X. (2018). Megahertz serial crystallography. Nature Communications, 9: 4025. doi:10.1038/s41467-018-06156-7.

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Wiedorn, M. O.1, Author
et, al.1, Author
Paulraj, L. X.2, 3, Author              
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1external, ou_persistent22              
2International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714              
3Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, ou_persistent22              

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 Abstract: The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

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Language(s): eng - English
 Dates: 2018-08-052018-08-212018-10-02
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-018-06156-7
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Title: Nature Communications
  Abbreviation : Nat. Commun.
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 9 Sequence Number: 4025 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723