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  Systems-Based Approaches to Unravel Networks and Individual Elements Involved in Apple Superficial Scald

Karagiannis, E., Tanou, G., Scossa, F., Samiotaki, M., Michailidis, M., Manioudaki, M., et al. (2020). Systems-Based Approaches to Unravel Networks and Individual Elements Involved in Apple Superficial Scald. Frontiers in Plant Science, 11: 8. doi:10.3389/fpls.2020.00008.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-E7A7-A Version Permalink: http://hdl.handle.net/21.11116/0000-0005-E7A8-9
Genre: Journal Article

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 Creators:
Karagiannis, Evangelos1, Author
Tanou, Georgia1, Author
Scossa, F.2, Author              
Samiotaki, Martina1, Author
Michailidis, Michail1, Author
Manioudaki, Maria1, Author
Laurens, Francois1, Author
Job, Dominique1, Author
Fernie, A. R.2, Author              
Orsel, Mathilde1, Author
Molassiotis, Athanassios1, Author
Affiliations:
1external, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: EXPRESSION PATTERN; GAS-CHROMATOGRAPHY; FREEZING TOLERANCE; GENE-EXPRESSION; COLD TOLERANCE; BARLEY GENE; FRUIT; ETHYLENE; METABOLITES; INHIBITIONPlant Sciences; apple fruit; ethylene inhibition; glutathione S-transferases; metabolites; ozone; proteomics; ripening; superficial scald;
 Abstract: Superficial scald is a major physiological disorder in apple fruit that is induced by cold storage and is mainly expressed as brown necrotic patches on peel tissue. However, a global view of the gene-protein-metabolite interactome underlying scald prevention/sensitivity is currently missing. Herein, we have found for the first time that cold storage in an atmosphere enriched with ozone (O-3) induced scald symptoms in 'Granny Smith' apple fruits during subsequent ripening at room temperature. In contrast, treatment with the ethylene perception inhibitor 1-methylcyclopropene (1-MCP) reversed this O-3-induced scald effect. Amino acids, including branched-chain amino acids, were the most strongly induced metabolites in peel tissue of 1-MCP treated fruits. Proteins involved in oxidative stress and protein trafficking were differentially accumulated prior to and during scald development. Genes involved in photosynthesis, flavonoid biosynthesis and ethylene signaling displayed significant alterations in response to 1-MCP and O-3. Analysis of regulatory module networks identified putative transcription factors (TFs) that could be involved in scald. Subsequently, a transcriptional network of the genes-proteins-metabolites and the connected TFs was constructed. This approach enabled identification of several genes coregulated by TFs, notably encoding glutathione S-transferase (GST) protein(s) with distinct signatures following 1-MCP and O-3 treatments. Overall, this study is an important contribution to future functional studies and breeding programs for this fruit, aiding to the development of improved apple cultivars to superficial scald.

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Language(s): eng - English
 Dates: 20202020
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000517413700001
DOI: 10.3389/fpls.2020.00008
 Degree: -

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Title: Frontiers in Plant Science
  Abbreviation : Front. Plant Sci.
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 11 Sequence Number: 8 Start / End Page: - Identifier: ISSN: 1664-462X
CoNE: https://pure.mpg.de/cone/journals/resource/1664462X