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  Development of an antioxidant assay to study oxidative potential of airborne particulate matter

Shapoury, P., Harner, T., Lammel, G., Lelieveld, S., Tong, H., & Wilson, J. (2019). Development of an antioxidant assay to study oxidative potential of airborne particulate matter. Atmospheric Measurement Techniques, 12(12), 6529-6539. doi:10.5194/amt-12-6529-2019.

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 Creators:
Shapoury, Pourya1, Author
Harner, Tom1, Author
Lammel, Gerhard2, Author              
Lelieveld, Steven2, Author              
Tong, Haijie2, Author              
Wilson, Jake2, Author              
Affiliations:
1external, ou_persistent22              
2Multiphase Chemistry, Max Planck Institute for Chemistry, Max Planck Society, ou_1826290              

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 Abstract: Oxidative potential is a measure of redox activity of airborne particulate matter (PM) and is often used as a surrogate to estimate one form of PM toxicity. The evaluation of oxidative potential in a physiologically relevant environment is always challenging. In this work, we developed a chromatographic method, employing an ultra-high-performance liquid chromatograph coupled to a triple-quadruple mass spectrometer, to determine the oxidative potential of PM from different sources. To this purpose, we measured the PM-induced oxidation of glutathione, cysteine, and ascorbic acid, and formation of glutathione disulfide and cystine, following PM addition to simulated epithelial lining fluids, which, in addition to the antioxidants, contained inorganic salts, a phospholipid, and proteins. The new method showed high precision and, when applied to standard reference PM, the oxidative potential was found to increase with the reaction time and PM concentration in the lung fluid. The antioxidant depletion rates were considerably higher than the rates found with the conventional dithiothreitol assay, indicating the higher sensitivity of the new method. The presence of the lung fluid inorganic species increased the oxidative potential determined through glutathione and cysteine, but showed an opposite effect with ascorbic acid, whereas the presence of proteins resulted in a moderate decrease in the oxidative potential. In the presence of PM2.5, glutathione and cysteine demonstrated similar depletion patterns, which were noticeably different from that of ascorbic acid, suggesting that cysteine could be used as an alternative to glutathione for probing oxidative potential.

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 Dates: 2019
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: ISI: 000502261600006
DOI: 10.5194/amt-12-6529-2019
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Title: Atmospheric Measurement Techniques
  Abbreviation : AMT
Source Genre: Journal
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Publ. Info: Göttingen : European Geosciences Union, Copernicus
Pages: - Volume / Issue: 12 (12) Sequence Number: - Start / End Page: 6529 - 6539 Identifier: ISSN: 1867-1381
CoNE: https://pure.mpg.de/cone/journals/resource/1867-1381