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  Size-Resolved Single-Particle Fluorescence Spectrometer for Real-Time Analysis of Bioaerosols: Laboratory Evaluation and Atmospheric Measurements

Zhang, M., Klimach, T., Ma, N., Könemann, T., Pöhlker, C., Wang, Z., et al. (2019). Size-Resolved Single-Particle Fluorescence Spectrometer for Real-Time Analysis of Bioaerosols: Laboratory Evaluation and Atmospheric Measurements. Environmental Science & Technology, 53(22), 13257-13264. doi:10.1021/acs.est.9b01862.

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 Creators:
Zhang, Minghui1, Author              
Klimach, Thomas2, Author              
Ma, Nan1, Author              
Könemann, Tobias1, Author              
Pöhlker, Christopher1, Author              
Wang, Zhibin1, Author              
Kuhn, Uwe1, Author              
Scheck, Nelli3, Author
Pöschl, Ulrich1, Author              
Su, Hang1, Author              
Cheng, Yafang1, Author              
Affiliations:
1Multiphase Chemistry, Max Planck Institute for Chemistry, Max Planck Society, ou_1826290              
2Particle Chemistry, Max Planck Institute for Chemistry, Max Planck Society, ou_1826291              
3external, ou_persistent22              

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 Abstract: Characteristic particle size, fluorescence intensity, and fluorescence spectra are important features to detect and categorize bioaerosols. A prototype size-resolved single-particle fluorescence spectrometer (S2FS) was developed to simultaneously measure aerodynamic diameters and fluorescence spectra. Emission spectra are dispersed in 512 channels from 370 to 610 nm, where a major portion of biological fluorescence emission occurs. The S2FS consists of an aerodynamic particle sizer and a fluorescence spectrometer with a 355 nm laser excitation source and an intensified charge-coupled device as the detector. Highly fluorescent particles, such as Ambrosia artemisiifolia pollen and Olea europaea pollen, can be distinguished by the S2FS on a single-particle level. For weakly fluorescent particles, fluorescence spectra can only be obtained by averaging multiple particles (between 100 and 3000) of the same kind. Preliminary ambient measurements in Mainz (Germany, central Europe) show that an emission peak at ∼ 440 nm was frequently observed for fluorescent fine particles (0.5-1 μm). Fluorescent fine particles accounted for 2.8% on average based on the number fraction in the fine mode. Fluorescent coarse particles (>1 μm) accounted for 8.9% on average based on the number fraction, with strongest occurrence observed during a thunderstorm and in the morning.

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 Dates: 2019
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: ISI: 000498279400030
DOI: 10.1021/acs.est.9b01862
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Title: Environmental Science & Technology
Source Genre: Journal
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Publ. Info: Easton, PA : American Chemical Society
Pages: - Volume / Issue: 53 (22) Sequence Number: - Start / End Page: 13257 - 13264 Identifier: ISSN: 0013-936X
CoNE: https://pure.mpg.de/cone/journals/resource/954921342157