hide
Free keywords:
SINGLE-STRANDED-DNA; REPLICATION PROTEIN-A; CHECKPOINT KINASE; BREAK
REPAIR; CHROMATIN; ACTIVATION; PHOSPHORYLATION; RECOMBINATION; DYNAMICS;
YEASTDNA damage checkpoint; Signal transduction; Double strand break; DNA end
resection; Cell cycle; Post-translational modification; Genome stability;
Abstract:
DNA damage occurs abundantly during normal cellular proliferation. This necessitates that cellular DNA damage response and checkpoint pathways monitor the cellular DNA damage load and that DNA damage signaling is quantitative. Yet, how DNA lesions are counted and converted into a quantitative response remains poorly understood. We have recently obtained insights into this question investigating DNA damage signaling elicited by single-stranded DNA (ssDNA). Intriguingly, our findings suggest that local and global DNA damage signaling react differentially to increasing amounts of DNA damage. In this mini-review, we will discuss these findings and put them into perspective of current knowledge on the DNA damage response.
