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  Shaping Giant Membrane Vesicles in 3D-Printed Protein Hydrogel Cages

Jia, H., Litschel, T., Heymann, M., Eto, H., Franquelim, H. G., & Schwille, P. (2020). Shaping Giant Membrane Vesicles in 3D-Printed Protein Hydrogel Cages. Small, 1906259. doi:10.1002/smll.201906259.

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 Creators:
Jia, Haiyang1, Author              
Litschel, Thomas1, Author              
Heymann, Michael1, Author              
Eto, Hiromune1, Author              
Franquelim, Henri G.1, Author              
Schwille, Petra1, Author              
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: UNILAMELLAR VESICLES; CELL; ORGANIZATION; CURVATURE; RECONSTITUTION; OSCILLATIONS; SEPARATION; TENSION; PHASES3D printing; bottom-up synthetic biology; hydrogels; membranes; Min system;
 Abstract: Giant unilamellar phospholipid vesicles are attractive starting points for constructing minimal living cells from the bottom-up. Their membranes are compatible with many physiologically functional modules and act as selective barriers, while retaining a high morphological flexibility. However, their spherical shape renders them rather inappropriate to study phenomena that are based on distinct cell shape and polarity, such as cell division. Here, a microscale device based on 3D printed protein hydrogel is introduced to induce pH-stimulated reversible shape changes in trapped vesicles without compromising their free-standing membranes. Deformations of spheres to at least twice their aspect ratio, but also toward unusual quadratic or triangular shapes can be accomplished. Mechanical force induced by the cages to phase-separated membrane vesicles can lead to spontaneous shape deformations, from the recurrent formation of dumbbells with curved necks between domains to full budding of membrane domains as separate vesicles. Moreover, shape-tunable vesicles are particularly desirable when reconstituting geometry-sensitive protein networks, such as reaction-diffusion systems. In particular, vesicle shape changes allow to switch between different modes of self-organized protein oscillations within, and thus, to influence reaction networks directly by external mechanical cues.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published online
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000516657400001
DOI: 10.1002/smll.201906259
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Project name : GRK2062, Molecular Principles of Synthetic Biology
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Funding organization : Deutsche Forschungsgemeinschaft (DFG)
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Grant ID : SFB 863
Funding program : -
Funding organization : DFG

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Title: Small
  Other : Small
Source Genre: Journal
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Publ. Info: Weinheim, Germany : Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: 1906259 Start / End Page: - Identifier: ISSN: 1613-6810
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000017440_1