English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Integrin-Mediated Focal Anchorage Drives Epithelial Zippering during Mouse Neural Tube Closure

Mole, M. A., Galea, G. L., Rolo, A., Weberling, A., Nychyk, O., De Castro, S. C., et al. (2020). Integrin-Mediated Focal Anchorage Drives Epithelial Zippering during Mouse Neural Tube Closure. DEVELOPMENTAL CELL, 52(3), 321-334. doi:10.1016/j.devcel.2020.01.012.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
1-s2.0-S1534580720300137-main.pdf (Any fulltext), 11MB
Name:
1-s2.0-S1534580720300137-main.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
open access article
License:
-

Locators

show

Creators

show
hide
 Creators:
Mole, Matteo A.1, Author
Galea, Gabriel L.1, Author
Rolo, Ana1, Author
Weberling, Antonia1, Author
Nychyk, Oleksandr1, Author
De Castro, Sandra C.1, Author
Savery, Dawn1, Author
Fässler, Reinhard2, Author              
Ybot-Gonzalez, Patricia1, Author
Greene, Nicholas D. E.1, Author
Copp, Andrew J.1, Author
Affiliations:
1external, ou_persistent22              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

Content

show
hide
Free keywords: DORSAL CLOSURE; MORPHOGENESIS; FORCES; NEURULATION; ACTIVATION; APOPTOSIS; ADHESION; FUSION; CRE
 Abstract: Epithelial fusion is a key process of morphogenesis by which tissue connectivity is established between adjacent epithelial sheets. A striking and poorly understood feature of this process is "zippering," whereby a fusion point moves directionally along an organ rudiment. Here, we uncover the molecular mechanism underlying zippering during mouse spinal neural tube closure. Fusion is initiated via local activation of integrin beta 1 and focal anchorage of surface ectoderm cells to a shared point of fibronectin-rich basement membrane, where the neural folds first contact each other. Surface ectoderm cells undergo proximal junction shortening, establishing a transitory semi-rosette-like structure at the zippering point that promotes juxtaposition of cells across the midline enabling fusion propagation. Tissue-specific ablation of integrin beta 1 abolishes the semi-rosette formation, preventing zippering and causing spina bifida. We propose integrin-mediated anchorage as an evolutionarily conserved mechanism of general relevance for zippering closure of epithelial gaps whose disturbance can produce clinically important birth defects.

Details

show
hide
Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: DEVELOPMENTAL CELL
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 52 (3) Sequence Number: - Start / End Page: 321 - 334 Identifier: ISSN: 1534-5807