English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Electrocoalescence of water-in-oil droplets with a continuous aqueous phase: implementation of controlled content release

Frey, C., Göpfrich, K., Pashapour, S., Platzman, I., & Spatz, J. P. (2020). Electrocoalescence of water-in-oil droplets with a continuous aqueous phase: implementation of controlled content release. ACS Omega, 5(13), 7529-7536. doi:10.1021/acsomega.0c00344.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-F643-A Version Permalink: http://hdl.handle.net/21.11116/0000-0006-09FD-4
Genre: Journal Article

Files

show Files
hide Files
:
ACSOmega_20_2020_7529.pdf (Any fulltext), 4MB
 
File Permalink:
-
Name:
ACSOmega_20_2020_7529.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
ACSOmega_20_2020_7529_Suppl.pdf (Supplementary material), 2MB
 
File Permalink:
-
Name:
ACSOmega_20_2020_7529_Suppl.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Frey, Christoph1, 2, Author              
Göpfrich, Kerstin1, 2, Author              
Pashapour, Sadaf1, 2, Author              
Platzman, Ilia1, 2, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Droplet-based microfluidics have emerged as an important tool for diverse biomedical and biological applications including, but not limited to, drug screening, cellular analysis, and bottom-up synthetic biology. Each microfluidic water-in-oil droplet contains a well-defined biocontent that, following its manipulation/maturation, has to be released into a physiological environment toward possible end-user investigations. Despite the progress made in recent years, considerable challenges still loom at achieving a precise control over the content release with sufficient speed and sensitivity. Here, we present a quantitative study in which we compare the effectiveness and biocompatibility of chemical and physical microfluidic release methods. We show the advantages of electrocoalescence of water-in-oil droplets in terms of high-throughput release applications. Moreover, we apply programmable DNA nanotechnology to achieve a segregation of the biochemical content within the droplets for the controlled filtration of the encapsulated materials. We envision that the developed bifunctional microfluidic approach, capable of content segregation and selective release, will expand the microfluidic toolbox for cell biology, synthetic biology, and biomedical applications.

Details

show
hide
Language(s): eng - English
 Dates: 2020-01-242020-03-232020
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acsomega.0c00344
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ACS Omega
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 5 (13) Sequence Number: - Start / End Page: 7529 - 7536 Identifier: ISSN: 2470-1343
CoNE: https://pure.mpg.de/cone/journals/resource/2470-1343