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  ELAV links paused Pol II to alternative polyadenylation in the Drosophila nervious system

Oktaba, K., Zhang, W., Lotz, T. S., Jun, D. J., Lemke, S. B., Ng, S. P., et al. (2015). ELAV links paused Pol II to alternative polyadenylation in the Drosophila nervious system. Molecular Cell, 57, 341-348. doi:10.1016/j.molcel.2014.11.024.

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Oktaba, Katarzyna1, Author
Zhang, Wei1, Author
Lotz, Thea Sabrina1, Author
Jun, David Jayhyun1, Author
Lemke, Sandra Beatrice1, Author
Ng, Samuel Pak1, Author
Esposito, Emilia1, Author
Levine, Michael1, Author
Hilgers, Valérie2, Author              
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1External Organizations, ou_persistent22              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243642              

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 Abstract: Alternative polyadenylation (APA) has been implicated in a variety of developmental and disease processes. A particularly dramatic form of APA occurs in the developing nervous system of flies and mammals, whereby various developmental genes undergo coordinate 3' UTR extension. In Drosophila, the RNA-binding protein ELAV inhibits RNA processing at proximal polyadenylation sites, thereby fostering the formation of exceptionally long 3' UTRs. Here, we present evidence that paused Pol II promotes recruitment of ELAV to extended genes. Replacing promoters of extended genes with heterologous promoters blocks normal 3' extension in the nervous system, while extension-associated promoters can induce 3' extension in ectopic tissues expressing ELAV. Computational analyses suggest that promoter regions of extended genes tend to contain paused Pol II and associated cis-regulatory elements such as GAGA. ChIP-seq assays identify ELAV in the promoter regions of extended genes. Our study provides evidence for a regulatory link between promoter-proximal pausing and APA.

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Language(s): eng - English
 Dates: 2015-01-22
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.molcel.2014.11.024
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Title: Molecular Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 57 Sequence Number: - Start / End Page: 341 - 348 Identifier: ISSN: 1097-2765
CoNE: https://pure.mpg.de/cone/journals/resource/954925610929