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  Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos

Löser, E., Latrielle, D., & Iovino, N. (2016). Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos. In Methods in Molecular Biology (pp. 23-36).

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Item Permalink: https://hdl.handle.net/21.11116/0000-0006-054E-E Version Permalink: https://hdl.handle.net/21.11116/0000-0008-A5E2-E
Genre: Book Chapter

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Löser et al..pdf (Publisher version), 422KB
 
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https://link.springer.com/protocol/10.1007%2F978-1-4939-6380-5_3 (Publisher version)
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 Creators:
Löser, Eva1, Author
Latrielle, Daniel1, Author
Iovino, Nicola1, Author           
Affiliations:
1Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243643              

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Free keywords: Chromatin immunoprecipitation, Drosophila embryos, ChIP-Seq
 Abstract: This protocol provides specific details on how to perform Chromatin immunoprecipitation (ChIP) from Drosophila embryos. ChIP allows the matching of proteins or histone modifications to specific genomic regions. Formaldehyde-cross-linked chromatin is isolated and antibodies against the target of interest are used to determine whether the target is associated with a specific DNA sequence. This can be performed in spatial and temporal manner and it can provide information about the genome-wide localization of a given protein or histone modification if coupled with deep sequencing technology (ChIP-Seq).

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Language(s): eng - English
 Dates: 2016
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4939-6380-5_28
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Title: Methods in Molecular Biology
Source Genre: Book
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Pages: - Volume / Issue: 1480 Sequence Number: - Start / End Page: 23 - 36 Identifier: -