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  Monosomes actively translate synaptic mRNAs in neuronal processes

Biever, A., Glock, C., Tushev, G., Ciirdaeva, E., Dalmay, T., Langer, J. D., et al. (2020). Monosomes actively translate synaptic mRNAs in neuronal processes. Science, 367(6477). doi:10.1126/science.aay4991.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-0931-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-0932-8
Genre: Journal Article

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 Creators:
Biever, Anne1, Author
Glock, Caspar1, Author
Tushev, Georgi1, Author
Ciirdaeva, Elena1, Author
Dalmay, Tushev1, Author
Langer, Julian D.1, 2, Author
Schuman, Erin M.1, 3, Author
Affiliations:
1Max Planck Institute of Biophysics, Max Planck Society, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, DE, ou_2068288              
2Max Planck Institute for Brain Research, Max Planck Society, Max-von-Laue-Str. 4, 60438 Frankfurt am Main, DE, ou_2461692              
3Max Planck Institute for Brain Research, Frankfurt, Germany, electronic address: erin.schuman@brain.mpg.de, ou_persistent22              

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 Abstract: To accommodate their complex morphology, neurons localize messenger RNAs (mRNAs) and ribosomes near synapses to produce proteins locally. However, a relative paucity of polysomes (considered the active sites of translation) detected in electron micrographs of neuronal processes has suggested a limited capacity for local protein synthesis. In this study, we used polysome profiling together with ribosome footprinting of microdissected rodent synaptic regions to reveal a surprisingly high number of dendritic and/or axonal transcripts preferentially associated with monosomes (single ribosomes). Furthermore, the neuronal monosomes were in the process of active protein synthesis. Most mRNAs showed a similar translational status in the cell bodies and neurites, but some transcripts exhibited differential ribosome occupancy in the compartments. Monosome-preferring transcripts often encoded high-abundance synaptic proteins. Thus, monosome translation contributes to the local neuronal proteome.

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Language(s): eng - English
 Dates: 2020-01-31
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: To accommodate their complex morphology, neurons localize messenger RNAs (mRNAs) and ribosomes near synapses to produce proteins locally. However, a relative paucity of polysomes (considered the active sites of translation) detected in electron micrographs of neuronal processes has suggested a limited capacity for local protein synthesis. In this study, we used polysome profiling together with ribosome footprinting of microdissected rodent synaptic regions to reveal a surprisingly high number of dendritic and/or axonal transcripts preferentially associated with monosomes (single ribosomes). Furthermore, the neuronal monosomes were in the process of active protein synthesis. Most mRNAs showed a similar translational status in the cell bodies and neurites, but some transcripts exhibited differential ribosome occupancy in the compartments. Monosome-preferring transcripts often encoded high-abundance synaptic proteins. Thus, monosome translation contributes to the local neuronal proteome.
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.aay4991
PMID: 32001627
 Degree: -

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Title: Science
  Other : Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 367 (6477) Sequence Number: - Start / End Page: - Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1