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  Continuous volumetric imaging via an optical phase-locked ultrasound lens

Kong, L., Tang, J., Little, J. P., Yu, Y., Lämmermann, T., Lin, C. P., et al. (2016). Continuous volumetric imaging via an optical phase-locked ultrasound lens. Nature methods, 12, 759-762. doi:10.1038/nmeth.3476.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0006-09C5-2 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-A58D-F
Genre: Journal Article

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https://www.nature.com/articles/nmeth.3476 (Publisher version)
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 Creators:
Kong, Lingjie1, Author
Tang, Jianyong1, Author
Little, Justin P.1, Author
Yu, Yang1, Author
Lämmermann, Tim2, Author           
Lin, Charles P.1, Author
Germain, Ronald N.1, Author
Cui, Meng1, Author
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1External Organizations, ou_persistent22              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_1565141              

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 Abstract: In vivo imaging at high spatiotemporal resolution is key to the understanding of complex biological systems. We integrated an optical phase-locked ultrasound lens into a two-photon fluorescence microscope and achieved microsecond-scale axial scanning, thus enabling volumetric imaging at tens of hertz. We applied this system to multicolor volumetric imaging of processes sensitive to motion artifacts, including calcium dynamics in behaving mouse brain and transient morphology changes and trafficking of immune cells.

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Language(s): eng - English
 Dates: 2016-06
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nmeth.3476
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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 12 Sequence Number: - Start / End Page: 759 - 762 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556